Tonicity response element binding protein associated with neuronal cell death in the experimental diabetic retinopathy

查看全文 作　　者：Seong-Jae [1,2]Kim;Hwajin [3,2]Kim;Jeongsook [3,2]Park;Inyoung [1]Chung;Hyug-Moo [4]Kwon;Wan-Sung [3,2]Choi;Ji-Myong [1,2]Yoo 机构地区：[1]Department of Ophthalmology,School of Medicine,Gyeongsang National University;[2]Institute of Health Science,Gyeongsang National University;[3]Department of Anatomy and Neurobiology,BK21 Biomedical Center,School of Medicine,Gyeongsang National University;[4]School of Nano-Bioscience and Chemical Engineering,Ulsan National Institute and Science and Technology 出　　处：《International Journal of Ophthalmology(English edition)》索引2014年第7卷第6期,共6页 基　　金：Supported by the Basic Research Program of the Korea Science & Engineering Foundation (No. 2009-0068732);the Basic Research Program of the National Research Foundation of Korea (No.2011-0020163);the Bio-Industry Technology Development Program funded by the Korea Institute of Planning & Evaluation for Technology in Food, Agriculture Forestry & Fisheries (No.112005-3);the BK21 Program and by the MRC program of KRF (R13-2005-012-01001-1) 摘　　要：AIM: To study the contribution of tonicity response element binding protein(Ton EBP) in retinal ganglion cell(RGC) death of diabetic retinopathy(DR).METHODS: Diabetes was induced in C57BL/6 mice by five consecutive intraperitoneal injections of 55 mg/kg streptozotocin(STZ). Control mice received vehicle(phosphate-buffered saline). All mice were killed 2mo after injections, and the extent of cell death and the protein expression levels of Ton EBP and aldose reductase(AR) were examined.RESULTS: The Ton EBP and AR protein levels and the death of RGC were significantly increased in the retinas of diabetic mice compared with controls 2mo after the induction of diabetes. Terminal deoxynucleotidyl transferase(Td T)-mediated d UTP nick end labeling(TUNEL)-positive signals co-localized with Ton EBP immunoreactive RGC. These changes were increased in the diabetic retinas compared with controls.CONCLUSION: The present data show that AR and Ton EBP are upregulated in the DR and Ton EBP may contribute to apoptosis of RGC in the DR. 关 键 词：aldose reductase DIABETES tonicity response element binding protein RETINOPATHY