Batroxobin reduces intracellular calcium concentration and inhibits proliferation of vascular smooth muscle cells

查看全文 作　　者：[1]宋清斌;[2]魏敏杰;[1]段志泉;[1]张海强;LB [3]Schwartz;[1]辛世杰

机构地区：[1]Department of Vascular Surgery,First Affiliated Hospital,China Medical University;[2]Department of Pharmacology,China Medical University;[3]Department of Vascular Surgery,University of Chicago 60637

出　　处：《Chinese Medical Journal》索引2004年第6期,共5页

基　　金：ThisstudywassupportedbytheNationalNaturalScienceFoundationofChina (No 3 0 0 0 0 163 ) 摘　　要：Background Batroxobin (BX),a serine protease used in defibrinogenation and thrombolysis,also has an effect on c-fos gene and growth factor. This study attempted to determine the effects of BX on the proliferation of vascular smooth muscle cells (VSMCs) and calcium metabolism. Methods VSMCs were treated with BX at concentrations of 0.1,0.3,or 1.0 mmol/L and cell numbers were determined at 0,24,48,and 72 hours. Intracellular calcium concentration ([Ca 2+ ]_i) was measured using direct fluorescence methods. Results BX was found to suppress proliferation of VSMCs in a dose-dependent fashion with inhibition rates of 18% and 31% by 48 and 72 hours,respectively. In addition,BX decreases basal [Ca 2+ ]_i significantly. The basal level in untreated cells was 162.7±33.8 nmol/L,and decreased to 131.5±27.7 nmol/L,128.3±28.5 nmol/L,and 125.6±34.3 nmol/L with the three concentrations of BX,respectively. Noradrenaline (NE)-induced [Ca 2+ ]_i stimulation was also attenuated by BX (0.1 mmol/L BX,20%±8% inhibition; 0.3 mmol/L BX,54%±11% inhibition; 1.0 mmol/L BX,62%±15% inhibition). The ability of NE to stimulate [Ca 2+ ]_i was attenuated in cultures in Ca 2+ -free medium,as was the ability of BX to blunt NE-induced stimulation. Conclusion These findings demonstrate that BX can effectively inhibit proliferation of VSMCs,probably by blocking the release and uptake of Ca 2+ ,thus influencing [Ca 2+ ]_i. 关键词：类凝血酶还原反应细胞内钙钙浓度血管平滑肌细胞细胞增殖抑制作用 VSMCS 去甲肾上腺素

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Batroxobin reduces intracellular calcium concentration and inhibits proliferation of vascular smooth muscle cells

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