Cloning and identification of NS5A TP2gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A

查看全文 作　　者：[3]QianYang;[2]JunCheng;[2]YanLiu;[2]YuanHong;Jian-[2]JunWang;Shu-[1]LinZhang

机构地区：[1]DepartmentofInfectiousDisease,FirstHospitalofXi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China;[2]GeneTherapyResearchCenter,InstituteofInfectiousDiseases,302HospitalofPLA,100XisihuanzhongRoad,Beijing100039,China;[3]DepartmentofInfectiousDisease,FirstHospitalofXi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China

出　　处：《World Journal of Gastroenterology》索引2004年第10卷第12期,共5页

基　　金：Supported by the National Natural Science Foundation of China,No.C03011402,No.C30070690 and the 9th Five-year Plan Period Research and Development Foundation of PLA,No.98D063 and the Start-up for Students Studying Overseas of PLA,No.98H038 and the 10th F 摘　　要：AIM: To clone, identify and study new NSSATP2 gene and its spliced variant transactivated by hepatitis C virus nonstructural protein 5A. METHODS: On the basis of subtractive cDNA library of genes transactivated by NS5A protein of hepatitis C virus, the coding sequence of new gene and its spliced variant were obtained by bioinformatics method. Polymerase chain reaction (PCR) was conducted to amplify/VSSATP2 gene. RESULTS: The coding sequence of a new gene and its spliced variant were cloned and identified successfully. CONCLUSION: A new gene has been recognized as the new target transactivated by HCV NS5A protein. These results brought some new clues for studying the biological functions of new genes and pathogenesis of the viral proteins. 关键词：NS5ATP2基因鉴定法无性繁殖丙型肝炎病毒无结构蛋白5A 聚合酶链

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Cloning and identification of NS5A TP2gene and its spliced variant transactivated by hepatitis C virus non-structural protein 5A

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