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Polymorphisms of interleukin-1B and interleukin-1 receptor antagonist genes in patients with chronic hepatitis B

查看全文 作　　者：Ping-[1]AnZhang;[1]YanLi;[1]PuXu;Jian-[2]MinWu 高影响力作者 机构地区：[1]DepartmentofLaboratoryScience,RenminHospitalofWuhanUniversity,Wuhan430060,HubeiProvince,China;[2]DepartmentofLaboratoryScience,AffiliatedUnionHospitalofTongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430030,HubeiProvince,China高影响力机构 出　　处：《World Journal of Gastroenterology》索引2004年第10卷第12期,共4页高影响力期刊 摘　　要：AIM:To investigate the relationships between polymorphisms of interleukin-lB (IL-1B) promoter region -511CLT and interleukin-1 receptor antagonist gene (IL-1RN) and susceptibility to chronic hepatitis B in Chinese population. METHODS: Genomic DNA was extracted from the peripheral blood of 190 patients with chronic hepatitis B and 249 normal controls and then subjected to polymerase chain reaction (PCR) amplification. The PCR products were digested by restriction endonuclease AvaI. The products of digestion were subjected to 20 g/L gel electrophoresis and ethidium bromide staining. RESULTS: The frequencies of IL-1B (-511) genotypes CC, CT and TT in patients with chronic hepatitis B were 23.7%, 49.5% and 26.8%, while 26.1%, 47.4% and 26.5% respectively in controls. The results showed that there was no significant difference in the frequencies of alleles or genotypes in IL-1B between patients with chronic hepatitis B and controls. The distributions of IL-1B (-511) genotype CC were significantly different between the two subgroups (HBV-DNA ≤1×10^3 copies/mL as subgroup I, HBV-DNA> 1×10^3 copies/mL as subgroup Ⅱ) of chronic hepatitis B (P=0.029). Only four of the five kinds of polymorphism (1/1, 1/2, 2/2 and 1/4) were found in this study. The frequencies of IL-1RN genotypes 1/1, 1/2, 2/2 and 1/4 were 88.9%, 9.0%, 0.5% and 1.6% in patients with chronic hepatitis B respectively, while were 81.1%, 16.9%, 0.4% and 1.6% respectively in controls. The frequencies of genotype1/2 and IL-1RN allele 2 in patients with chronic hepatitis B were lower than those in controls (P=0.016 and P=0.029, respectively). CONCLUSION: There is an association between polyrnorphisms of the promoter region -511C/T of IL-1B and IL-1RN intron 2 and chronic hepatitis B virus infection. SubJects with IL-1RN 2 may be resistant to HBV infection, and IL-1B(-511) geNotype CC is closely related with HBV-DNA replication, which gives some new clues to the study of pathogenesis of chronic hepatitis B. 关 键 词：白细胞间介素-1B 多态性 受体 基因 慢性乙型肝炎 PCR
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