Transgene for growth hormone in common carp(Cyprinus carpio L.)promotes thymus development

查看全文 作　　者：GUO [1]Qionglin;WANG [1]Yaping;JIA [1]Weizhang;ZHU [1]Zuoyan

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology,Insti-tute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China

出　　处：《Chinese Science Bulletin》索引2003年第48卷第16期,共7页

基　　金：supported by the State High-Techmology Program and the Special Funds of the Major State Basic Research of China(Grant Nos.2001AA212281 and 2001CB109006);the National Natural Science Foundation of China(Grant Nos.301 30050 and 30070588). 摘　　要：The transgenic carp were produced by micro-injection of CAgcGHc into the fertilized eggs. Observation of the thymus development between the transgenics and non- transgenic controls was carried out. The thymus of one-year- old transgenics F1 showed a great increase in both size and weight. The unilateral thymus of the transgenics weighed from 190 to 295 mg with average 218.6 mg, whereas the uni-lateral thymus of the controls weighed 20—81 mg with av-erage 42.5 mg; i.e. the thymus weight in the transgenics was 5.14 fold over that in the controls. The index of thymus/body weight in the transgenics was 2.97 fold over the controls. Light microscopy observation indicated that the thymus of the transgenics well developed with the thickened outer re-gion and compactly arranged thymocytes, while the thymus in the controls were degenerating with the thinned outer region, scattered thymocytes and groups of fatty cells. Fur-ther analysis with the electron microscopy revealed that pro-liferous cells in the transgenics were mainly small lympho-cytes and no pathological changes were found. The results confirmed that the 揂ll-fish?GH-transgene promotes thy-mus development and thymocyte proliferation, and retards thymus degeneration. The study has laid a foundation for further analysis of the immunobiological function in GH- transgenic carp. 关键词：生长激素转基因鲤鱼受精卵胸腺发育显微镜观察方法胸腺细胞电子显微镜淋巴细胞变化病态变化

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Transgene for growth hormone in common carp(Cyprinus carpio L.)promotes thymus development

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