Proteomic response analysis of endothelial cells of human coronary artery to stimulation with carbachol

查看全文 作　　者：[1]MinYU;Dong-[1]meiCHEN;[2]GangHU;[1]HaiWANG

机构地区：[1]BeijingInstituteofPharmacologyandToxicology,Beijing100850,China;[2]DepartmentofPharmacologyandNeurobiology,NanjingMedicalUniversity,Nanjing210029,China

出　　处：《Acta Pharmacologica Sinica》索引2004年第25卷第9期,共7页

基　　金：ProjectsupportedbyaresearchgrantfromStateKeyBasicResearchandDevelopmentfromtheMinistryofScienceandTechnologyofChina(G1998051112) 摘　　要：AIM: To identify the molecular basis of the endothelial target for acetylcholine (ETA). METHODS: Proteomic methods were used to monitor changes in protein expression in the first 10h following the stimulation of human coronary endothelial cells with carbachol 100μmol/L. Thirty proteins showing the largest changes were identified by mass spectrometry. RESULTS: Based on analysis with Image Master 2-D Elite software, about 623 protein spots were detected in control cells and 825 protein spots in carbachol-treated cells, the matching rate was 68.1%. Among all the detected spots, 39 were up-regulated and 29 were down-regulated, showing detectable changes varied from 1.7-3.8 folds. Forty one spots in the peptide mass fingerprints were successfully obtained. The most interesting feature was that all the four newly synthesized proteins belonged to the heat shock protein family.CONCLUSION: These identified proteins played key roles in the molecular mechanism of ETA. 关键词：分解反应蛋白质细胞人类冠状动脉刺激作用氨甲酰胆碱蛋白质鉴定电泳二维凝胶体 ETA

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Proteomic response analysis of endothelial cells of human coronary artery to stimulation with carbachol

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