Growth inhibition and apoptosis induction of tanshinone Ⅱ-A on human hepatocellular carcinoma cells

查看全文 作　　者：Shu-LanYuan Yu-QuanWei Xiu-JieWang FeiXiao Sheng-FuLi [1]JieZhang

机构地区：[1]KeyLaboratoryofBiotherapyofHumanDiseases,MinistryofEducationofChinaandCancerCenter,WestChinaHospital,WestChinaMedicalSchool,SichuanUniversity,Chengdu610041,SichuanProvince,China

出　　处：《World Journal of Gastroenterology》索引2004年第10卷第14期,共5页

基　　金：Supported by the Science Foundation of the Ministry of Health of China,No.96-1-240 and the Applied Basic Research Programs of Science and Technology Commission Foundation of Sichuan Province,No.2000-135 摘　　要：AIM: To evaluate the effects of tanshinone II-A on inducing growth inhibition and apoptosis of human hepatocellular carcinoma (HCC) cells,METHODS: The human hepatocellular carcinoma cell line SMMC-7721 was used for the study. The cells were treated with tanshinone II-A at different doses and different times.Cell growth and proliferation were measured by MTT assay,cell count and colony-forming assay. Apoptosis induction was detected by microscopy, DNA ladder electrophoresis and flow cytometry.RESULTS: In MTT assay, the inhibitory effect became gradually stronger with the passage of time, 24, 48, 72and 96 h after treatment with tanshinone II-A, and the most significant effect was observed at 72 h. On the other hand, the increase of doses (0.125, 0.25, 0.5, 1.0 mg/L tanshinone II-A) resulted in enhanced inhibitory effect.The growth and proliferation of SMMC-7721 cells were obviously suppressed in a dose- and time-dependent manner. The results of cell count were similar to that of MTT assay. In colony-forming assay, the colony-forming rates were obviously inhibited by tanshinone II-A. In tanshinone II-A group, the morphology of cellular growth inhibition and characteristics of apoptosis such as chromatin condensation, crescent formation, margination and apoptotic body were observed under light and transmission electron microscopes. DNA ladder of cells was presented in electrophoresis. The apoptosis index (AI) was 16.9% (the control group was 4.6%) in flow cytometry. The cells were arrested in G0/G1 phase, and the expressions of apoptosis-related genes bd-2 and c-myc were down-regulated and fas, bax, p53 up-regulated.CONCLUSION: Tanshinone II-A could inhibit the growth and proliferation of HCC cell effectively in vitro by apoptosis induction,which was associated with up-regulation of fas, p53, bax,expression and down-regulation of bcl-2 and c-myc. 关键词：生长抑制作用细胞调亡感应现象丹参酮Ⅱ-A 肝细胞癌肿瘤 HCC

相关文献

参考文献（28）
引证文献（33）
耦合文献（97）

参考文献(28)

引证文献(33)

耦合文献(97)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Growth inhibition and apoptosis induction of tanshinone Ⅱ-A on human hepatocellular carcinoma cells

参考文献(28)

引证文献(33)

耦合文献(97)

用户提示