维普中文期刊产品整合服务

Cryodamage to plasma membrane integrity in head and tail regions of human sperm

查看全文 作　　者：Wei-Jie ZHU;Xue-Gao LIU Center for Reproductive Immunology Research, Jinan University, Guangzhou 510632, China 高影响力作者 出　　处：《Asian Journal of Andrology》索引2000年第2卷第2期,共4页高影响力期刊 摘　　要：Aim: To investigate the effect of cryopreservation on the plasma membrane integrity in the head and tail regions of individual sperm, and the relationship between intact cryopreserved sperm and its motility and zona-free hamster oocyte penetration rate. Methods: The eosin Y exclusion and the hypoosmotic swelling tests were combined to form a single test (HOS-EY test) to identify the spermatozoa with four types of membrane integrity. Results: After cryopreservation, there was a marked decline in the percentage of spermatozoa with Type IV membrane integrity (head membrane intact/tail membrane intact), and a significant increase in those with Type Ⅰ (head membrane damaged/tail membrane damaged) and Type Ⅲ (head membrane damaged/tail membrane intact) membrane integrity (n = 50, P<0.01). The value of Type Ⅲ integrity had a wide range of variability, whereas Type II (head membrane intact/tail membrane damaged) was uncommon after thawing. A high correlation was observed between the percentage of Type Ⅳ integrity and sperm motility ( n = 50, r = 0.74, P < 0.01 ). However, the values of Type IV integrity were usually lower than those of post-thaw motility in most cryopreserved samples. The value of Type IV integrity did not correlate with the sperm penetration rate (n =25, r=0.22, P>0.05). Conclusion: (1) The HOS-EY test has the advantage of showing four patterns of membrane integrity in individual spermatozoon; (2) Cryopreservation causes a significant membrane rupture in the head and tail regions of spermatozoa; Type IT[ is the main transitional state of membrane cryodamage; (3) Cryodamage to head and tail membrane may occur independently; the presence of an intact tail membrane does not necessarily indicate the intactness of head membrane. (4) Intact membranes am closely related to postthaw motility, but do not reflect the fertilizing potential. 关 键 词：冷冻伤 细胞膜 完整性 精子头部 精子尾部 精液 低温贮藏 超活体着色 低渗膨胀作用
相关文献

参考文献(1)

引证文献(14)

耦合文献(4)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费