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Antioxidant Effects of Dehydroepiandrosterone Are Related to Up-regulation of Thioredoxin in SH-SY5Y Cells

查看全文 作　　者：[1]JingGAO;Hui-[1]YingSUN;Zeng-[1]RongZHU;[1]ZhenDING;[2]LiZHU 高影响力作者 机构地区：[1]SchoolofMedicine,SchoolofLifeSciences,NanjingUniversityNanjing210093,China;[2]InstituteofNauticalMedicine,NantongUniversity,Nantong226001,China高影响力机构 出　　处：《Acta Biochimica et Biophysica Sinica》索引2005年第37卷第2期,共7页高影响力期刊 摘　　要：Neuroprotective effects of dehydroepiandrosterone (DHEA) have been shown to be associated with its antioxidant properties, but the mechanisms remain unknown. Considering that the thioredoxin (Trx)system, an important cellular redox modulation system, changes under oxidative stress and could exert protective effects, the relationship between the antioxidant effects of DHEA and the Trx system regulation was explored. Using MTT assay and morphological observation, the effects of DHEA in the model of H2O2-induced oxidative stress in SH-SY5Y cells were analyzed, then RT-PCR and Western blot assay were used to detect the alteration in mRNA and protein level of Trx. The results showed that a pre-treatment of DHEA (10-100 nM) protected cells against the toxicity induced by H2O2 in a dose-dependent manner, which could be confirmed in morphological observation by phase-contract microscope. In addition, Trx mRNA transcription was inhibited by H2O2 (300 μM), which could be reversed by the pre-administration of DHEA in various concentrations (0.1-100nM). Western blot assay confirmed that protein level of Trx could be elevated by the pre-treatment of DHEA (10-100nM) with the exposure of H2O2, Taken together, these data suggest that DHEA may be useful in treating age-related neurodegenerative diseases based on its up-regulating effects on an antioxidant and neuroprotective protein thioredoxin, a substrate in the Trx redox system. 关 键 词：抗氧化剂 脱氢异雄甾酮 硫氧还蛋白 SH-SY5Y细胞 过氧化氢 神经组织 保护作用
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