Programmed cell death in Laminaria japonica(Phaeophyta) tissues infected with alginic acid decomposing bacterium

查看全文 作　　者：[1]WANGGaoge;[1]LINWei;[2]ZHANGLijing;[1]YANXiaojun;[1]DUANDelin

机构地区：[1]InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China;[2]YellowSeaFisheriesResearchInstitute,ChineseAcademyofFisherySciences,Qingdao266071,China

出　　处：《Progress in Natural Science:Materials International》索引2004年第14卷第12期,共6页

基　　金：SupportedbytheMajorStateBasicResearchDevelopmentProgramofChina(GrantNo.G1999012004)andShangdongScienceandTechonologyCommittee(GrantNo.20032418) 摘　　要：TdT mediated dUTP biotin nick end labeling (TUNEL) is a sensitive and valid method for detecting DNA cleavage in programmed cell death (PCD). Using this method, DNA cleavage was observed in Laminaria japonica sporophytic tissues, which were infected with alginic acid decomposing bacterium. It was found that DNA cleavage occurred 5 min after the infection, the fragments with 3′ OH groups of cleaved nuclear DNA increased with time of infection and spread from the infection site. Although no typical DNA ladder (200 bp/180 bp) was detected by routine agarose gel electrophoresis, the cleavage of nuclear DNA fragments of 97~48.5 kb could be detected by pulsed field gel electrophoresis (PFGE). By using CaspGLOW TM fluorescein active caspase 3 staining method, caspase 3 activity has been detected in response to the infection of alginic acid decomposing bacterium. Our results are similar to the observations in hypersensitive response (HR) of higher plant, suggesting that the rapid cell death of L. japonica infected by alginic acid decomposing bacterium might be involved in PCD, and indicating that the occurrence of PCD is an active defense process against the pathogen’s infection. 关键词：昆布属植物褐藻门褐藻酸细菌分解过敏反应程序细胞死亡

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Programmed cell death in Laminaria japonica(Phaeophyta) tissues infected with alginic acid decomposing bacterium

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