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Proteomic analysis of nuclear matrix proteins during arsenic trioxide induced apoptosis in leukemia K562 cells

查看全文 作　　者：WANGZi-[1]hui;[1]YUDing;[2]CHENYan;HAOJian-[1]zhong 高影响力作者 机构地区：[1]BeijingUniversity,ShenzhenHospital,Shenzhen518036,China;[2]DepartmentofHematology,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,China高影响力机构 出　　处：《Chinese Medical Journal》索引2005年第2期,共5页高影响力期刊 基　　金：ThisstudywassupportbytheNationalNaturalScienceFoundationofChina(No 30271672) 摘　　要：Background Arsenic trioxide (As2O3) has been identified as a very potent antiacute leukemic agent However its role in apoptosis needs to be elucidated As2O3 interferes with the proliferation and survival of tumor cells via a variety of mechanisms Drugtarget interactions at the level of nuclear matrix (NM) may be critical events in the induction of cell death by As2O3 This study dealt with As2O3-target interactions at the level of NM in chronic myelogenous leukemia cell line K562 by proteomics Methods K562 cells were cultured in MEM and treated with different concentrations of As2O3 The nuclear matrix proteins were analyzed by highresolution twodimensional gel electrophoresis and computerassisted image analysis Results As2O3 significantly inhibited the growth of chronic myelogenous leukemia cell line K562 at low concentrations While more than 200 protein spots were shared among the nuclear matrices, about 18 distinct spots in the nuclear matrices were found characteristic for As2O3 treated cells Conclusions: As2O3 induces apoptosis in K562 cells in a dose and timedependent manner Our results demonstrated that for the detection of the onset of apoptosis, the alteration in the composition of nuclear matrix proteins was a more sensitive indicator than nucleosomal DNA fragmentation test These results indicated that As2O3 might be clinically useful in the treatment of chronic myelogenous leukemia. The changes of nuclear matrix proteins in the treated cells can be used as a useful indicator for this 关 键 词：蛋白质ο 核矩阵蛋白 三氧化砷 诱导作用 细胞凋亡 白血病 K562细胞 血液疾病
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