维普中文期刊产品整合服务

Therapeutic effect of autologous dendritic cell vaccine on patients with chronic hepatitis B: A clinical study

查看全文 作　　者：[1]MinChen;Yong-[2]GuoLi;Da-[2]ZhiZhang;Zhi-[2]YiWang;Wei-[2]QunZeng;Xiao-[2]FengShi;[2]YuanGuo;Shu-[2]HuaGuo;[2]HongRen 高影响力作者 机构地区：[1]theResearchInstituteforViralHepatitis,ChongqingUniversityofMedicalSciences,Chongqing400010,China;[2]DiseaseCenterofChongqingCity,theSecondAffiliatedHospitalofChongqingUniversityofMedicalSciences,Chongqing400010,China高影响力机构 出　　处：《World Journal of Gastroenterology》索引2005年第11卷第12期,共3页高影响力期刊 摘　　要：AIM: To investigate the therapeutic effect of autologous HBsAg-loaded dendritic cells (DCs) on patients with chronic hepatitis B.METHODS: Monocytes were isolated from fresh peripheral blood of 19 chronic HBV-infected patients by Ficoll-Hypaque density gradient centrifugation and cultured by plastic-adherence methods. DCs were induced and proliferated in the culture medium with recombinant human granulocyte-macrophage-colony- stimulating factor (rhGM-CSF) and human interleukin-4 (rhIL-4). DCs pulsed with HBsAg for twelve hours were injected into patients subcutaneously twice at intervals of two weeks. Two patients received 100 mg oral lamivudine daily for 12 mo at the same time. HBV-DNA and viral markers in sera of patients were tested every two months.RESULTS: By the end of 2003, 11 of 19 (57.9%) patients had a clinical response to DC-treatment. HBeAg of 10(52.6%) patients became negative, and the copies of HBVDNA decreased 101.77±2.39 averagely (t = 3.13, P<0.01).Two cases co-treated with DCs and lamivudine had a complete clinical response. There were no significant differences in the efficient rate between the cases with ALT level lower than 2xULN and those with ALT level higher than 2xULN before treatment (χ2 = 0.0026).CONCLUSION: Autologous DC-vaccine induced in vitro can effectively suppress HBV replication, reduce the virus load in sera, eliminate HBeAg and promote HBeAg/antiHBe transformation. Not only the patients with high serum ALT levels but also those with normal ALT levels can respond to DC vaccine treatment, and the treatment combining DCs with lamivudine can eliminate viruses more effectively. 关 键 词：自体树状细胞 疫苗接种 慢性乙型肝炎 治疗方法
相关文献

参考文献(14)

引证文献(42)

耦合文献(159)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费