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Genetic diversity of Chinese summer soybean germplasm revealed by SSR markers

查看全文 作  者:XIE Hua1,2, GUAN Rongxia1, CHANG Ruzhen1 & QIU Lijuan1* 1. Institute of Crop Germplasm Resources, Chinese Academy of Agri- cultural Sciences/Key Laboratory of Crop Germplasm & Biotechnol- ogy, Ministry of Agriculture, Beijing 100081, China;2. Beijing Agro-biotechnology Research Center, Beijing 100089, China 高影响力作者 出  处:《Chinese Science Bulletin》索引2005年第50卷第6期,共10页高影响力期刊 基  金:This work was supported by the National Basic Research Program of China(Grants Nos.G1998010203 and 2004CB117203). 摘  要:There are abundant soybean germplasm in China. In order to assess genetic diversity of Chinese sum- mer soybean germplasm, 158 Chinese summer soybean ac- cessions from the primary core collection of G. max were used to analyze genetic variation at 67 SSR loci. A total of 460 alleles were detected, in which 414 and 419 alleles oc- curred in the 80 Huanghuai and the 78 Southern summer accessions, respectively. The average number of alleles per locus was 6.9 for all the summer accessions, and 6.2 for both Huanghuai and Southern summer accessions. Marker diver- sity (D) per locus ranged from 0.414 to 0.905 with an average of 0.735 for all the summer accessions, from 0.387 to 0.886 with an average of 0.708 for the Huanghuai summer acces- sions, and from 0.189 to 0.884 with an average of 0.687 for the Southern summer accessions. The Huanghuai and Southern summer germplasm were different in the specific alleles, allelic-frequencies and pairwise genetic similarities. UPGMA cluster analysis based on the similarity data clearly separated the Huanghuai from Southern summer soybean accessions, suggesting that they were different gene pools. The results indicate that Chinese Huanghuai and Southern summer soybean germplasm can be used to enlarge genetic basis for developing elite summer soybean cultivars by ex- changing their germplasm. 关 键 词:遗传多样性 大豆 胚质 农作物 NDA 核酸 基因
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