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Establishment of a Core Collection for the Chinese annual wild soybean (Glycine Soja)

查看全文 作　　者：[1]ZHAOLimei;[2]DONGYingshan;[3]LIUBao;[3]HAOShui;[4]WANGKejing;[5]LIXianghua 高影响力作者 机构地区：[1]InstituteofGeneticsandCytology,NortheastNormalUniversity,Changchun130024,China//TheNationalCentreofPlantTransgenicResearch&Commercializa-tion,JilinAcademyofAgriculturalSciences,Gongzhuling136100,China;[2]TheNationalCentreofPlantTransgenicResearch&Commercializa-tion,JilinAcademyofAgriculturalSciences,Gongzhuling136100China//SchoolofLifeSciences,JilinUniversity,Changchun130021,China;[3]InstituteofGeneticsandCytology,NortheastNormalUniversity,Changchun130024,China;[4]InstituteofCropGermplasmResources,TheChineseAcademyofAgriculturalSciences,Beijing100081,China;[5]InstituteofCropGermplasmResources,TheChineseAcademyofAgriculturalSciences,Beijing100081,China高影响力机构 出　　处：《Chinese Science Bulletin》索引2005年第50卷第10期,共8页高影响力期刊 基　　金：This study was supported by the National Natural Science Foundational of China(Grant Nos.30370890&30490250);National Transgenic Plant Grant(No.JY03-B-16);the National Funds for Distinguished Young Scholars of China(No.30225003). 摘　　要：With more than 6000 accessions collected from across China, the Chinese National Crop Gene Bank (CNCGB) holds the largest collection of annual wild soybean (Glycine Soja) germplasm in the world. To facilitate the management and utilization of these germplasm collections, it is important to establish a Core Collection. This study compares five sampling strategies, namely random, constant, proportional, logarithmic and genetic diversity-based, to establish a Core Collection for the annual wild soybean germplasm. Among the strategies evaluated, the genetic di- versity-based was found to be the simplest and most efficient. Using hierarchical classification and cluster analysis, in the genetic diversity diversity-based strategy, 652 accessions, accounting for 10.65% of the total 6172 accessions, were se- lected out to represent the total accessions. The established Core Collection has the following features: (i) the 21 de- scriptors observed in the entire collection were compared by the Core Collection, all 18 quality characters of entire collec- tion were preserved by core collection, and coincidence rate of average was 98.4%; (ii) the variant of 13 descriptors of the two collections was very similar, with the coincidence index being 0.96; (iii) the coincidence rate of genetic diversity be- tween the two collections was 81.38% DNA alleles; (iv) the geographic distribution pattern of core collection was the same as the entire collection; (v) molecular marker analysis by 20 SSR primer pairs on 299 accessions showed that the Core Collection covered 83.64% of the entire collection. It thus is concluded that the established Core Collection is rep- resentative and will be a valuable entry point for better evaluation and more efficient utilization of the genetic re- sources available in the annual wild soybean germplasm bank. 关 键 词：一年生植物 大豆 遗传多样性 取样技术
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