Genes transactivated by hepatitis C virus core protein, a microarray assay

查看全文 作　　者：[3]MinLiu;Shu-[1]LinZhang;[2]JunCheng;[2]YanLiu;[2]LinWang;[2]QingShao;[2]JianZhang;Shu-[1]MeiLin

机构地区：[1]DepartmentofInfectiousDiseases,TheFirstAffiliatedHospital,MedicalCollege,Xi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China;[2]GeneTherapyResearchCenter,InstituteofInfectiousDiseases,The302HospitalofPLA,Beijing100039,China;[3]DepartmentofInfectiousDiseases,TheFirstAffiliatedHospital,MedicalCollege,Xi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China

出　　处：《World Journal of Gastroenterology》索引2005年第11卷第22期,共6页

基　　金：Supported by the National Natural Science Foundation of China, No. 39970674 摘　　要：AIM: To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection.METHODS: Reverse transcribed cDNA was subjected tomicroarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods.RESULTS: The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene namedHCTP4 was cloned with molecular biological method in combination with bioinformatics method.CONCLUSION: HCV core is a potential transactivator.Microarray is an efficient and convenient method for analysis of differentially expressed genes. 关键词：丙型肝炎基因表达肝炎病毒核心蛋白实验室检查

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Genes transactivated by hepatitis C virus core protein, a microarray assay

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