Effect of vector-expressed shRNAs on hTERT expression

查看全文 作　　者：[1]YingGuo;[2]JunLiu;Ying-[2]HuiLi;Tian-[3]BaoSong;[1]JingWu;Cai-[1]XiaZheng;Cai-[2]FangXue

机构地区：[1]DepartmentofObstetricsandGynecology,SecondHospitalofXi'anJiaotongUniversity,Xi'an710001,ShaanxiProvince,China;[2]DepartmentofEtiology,FourthMilitaryMedicalUniversity,Xi'an710033,ShaanxiProvince,China;[3]DepartmentofHistologyandEmbryology,MedicalSchoolofXi'anJiaotongUniversity,Xi'an710061,ShaanxiProvince,China

出　　处：《World Journal of Gastroenterology》索引2005年第11卷第19期,共4页

基　　金：Supported by the Shaanxi Province Natural Science Foundation,No. 2003C217 摘　　要：AIM: To study the effect of short hairpin RNAs (shRNAs)expressed from DNA vector on hTERT expression.METHODS: Oligonucleotides coding for four shRNAs against hTERT were cloned into a mammalian shRNA expressionvector pUC18U6 to form pUC18U6ht1-4, which were thenintroduced into HepG2 cells by using liposome-mediated transfection. HepG2 cells transfected by pUC18U6 and pUC18U6GFPsir, which expressed shRNA against green fluorescent protein (GFP), were used as controls. hTERT mRNA in the transfected cells were quantified by using real-time fluorescent RT-PCR.RESULTS: Among the four shRNAs against hTERT, two decreased the hTERT mRNA level. Compared with the controls, pUC18U6ht which expressed the two shRNAs reduced hTERT mRNA by 39% and 49% (P<0.05).CONCLUSION: hTERT expression is inhibited by the shRNAs expressed from the DNA vector. 关键词：shRNAs HTERT 基因表达 pUC18U6 基因克隆荧光蛋白质

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Effect of vector-expressed shRNAs on hTERT expression

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