维普中文期刊产品整合服务

Screening for Y chromosome microdeletions in idiopathic and nonidiopathic infertile men with varicocele and cryptorchidism

查看全文 作　　者：SONG Ning-[1]hong;WU Hong-[1]fei;ZHANG [1]Wei;ZHUO Zuo-[2]min;QIAN Li-[1]xing;HUA Li-[1]xing;GUO [3]Lin;FENG Ning-[1]han 高影响力作者 机构地区：[1]Department of Urology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China;[2]Key Laboratory of Reproductive Medicine of Jiangsu Province, Nanjing Medical University, Nanjing 210029, China;[3]Model Animal Research Center, Nanjing University, Nanjing 210032, China高影响力机构 出　　处：《Chinese Medical Journal》索引2005年第17期,共6页高影响力期刊 基　　金：The work was supported by the '135' Foundation of JiangsuProvince (No.0151). 摘　　要：Background Cytogenetic and molecular studies of azoospermic and oligozoospermic males have suggested thepresence of azoospermia factors (AZF) in the Y chromosome.Deletion in AZF regions has been reported todisrupt spermatogenesis and cause infertility.Several candidate genes responsible for spermatogenesis have beenidentified in this region and some of them are thought to be functional in human spermatogenesis.And wereported clinical and molecular studies of Ychromosome microdeletions in Chinese.This study aimed atassessingthe frequency of microdeletions in Chinese men with idiopathic and nonidiopathic infertility problems anddicussing the clinical significance of the AZF region.Methods In this study, we screened 143 infertile men (62 with idiopathic infertilitas and 81 withnonidiopathic infertilitas), in whom karyotype, sperm count, hormonal parameters and fine needle aspirationcytology were evaluated.Genomic DNA was extracted from the peripheral leukocytes.Molecular analysis wasperformed by two multiplexpolymerase chain reactions (PCR) using a set of a sequence tagged sites (STS) from3 different regions of the Y chromosome: AZFa (sY84, sY86), AZFb (sY127, sY134), AZFc (sY254,sY255).Results Nineteen point four percent of idiopathic males (12/62, 19.4%) had microdeletions of either theAZFa, AZFb, AZFc or AZFb +c region.Significantly, a high frequency of microdeletions (9/81, 11.1%) wasfound in nonidiopathic patients with varicocele and cryptorchidism.No deletions were found in healthy fertilemen.There were no significant differences in the localization and extent of deletions between idiopathic andnonidiopathic patients.Conclusions The knowledge of the presence of these deletions in idiopathic and nonidiopathic cases isimportant to understand the prognosis, better management and counsel these patients accordingly.Furthermore,a more extended screening for Y chromosome microdeletions in idiopathic and nonidiopathic men, particularlycandidates for intracytoplasmic sperm injection, is recommended. 关 键 词：Y染色体 精索静脉曲张 隐睾病 男性 病理机制
相关文献

参考文献(2)

引证文献(12)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费