Anti-enrofloxacin Antibody Production by Using Enrofloxacin-screened HSA as an Immunogen

查看全文 作　　者：LIU Chune LIN Hong CAO Limin JIANG [1]Jie 机构地区：[1]Aquatic Products Safety Laboratory, Ocean University of China, Qingdao 266003, P.R. China 出　　处：《Journal of Ocean University of China》索引2005年第4卷第3期,共5页 基　　金：supported by the National Natural Science Fundation of China(No.30400336);Science and Technology Project of Qingdao(03-1-NSH-2-2). 摘　　要：A two-step zero-length cross-linking procedure using active esters was successfully adopted for conjugating en-rofloxacin (EF) to human serum albumin (HSA). The derived conjugate was characterized by UV spectrum and then usedfor immunization of BALB/C mice. In enzyme-linked immunosorbent assay (ELISA) and competitive inhibition ELISA ex-periments, the derived antiserum exhibited high antibody titer (greater than 1: 250 000) as well as varied cross-reactivity(from 97.8% to 161.7%) to three analogs of EF belonging to fluoroquinolones family. But over the concentration rangestudied, no significant cross-reactivity was observed to other group of antibiotics (chloramphenicol, oxytetracycline, sul-phamethoxazole and nysfungin). It was confirmed that the synthesized immunogen was highly antigenic and elicited specificantibody responses in BALB/C mice against EF. 关 键 词：免疫原 抗体 HSA 抗血清 抗生素 土霉素