Preparation of ChlL-2 and IBDV VP2 Fusion Protein by Baculovirus Expression System

查看全文 作　　者：Yan [1,2]Liu;Yongwei [2]Wei;Xiaofeng [3]Wu;Lian [2]Yu

机构地区：[1]Department of Biomedical Engineering, Zhejiang University, Hangzhou,310027, China;[2]Zhejiang Province Key Lab of Preventive Veterinary Medicine, China;[3]College of Animal Sciences, Zhejiang University, Huajiachi Campus,Hangzhou 310029, China

出　　处：《Cellular & Molecular Immunology》索引2005年第2卷第3期,共5页

基　　金：This work was supported in part by the grants from the National High Technology Research and Development Program of China(No.2004BA757C);the Key Project of Zhejiang Province(NO.011102465). 摘　　要：This study aims to produce an effective subunit vaccine against infectious bursal disease virus (IBDV). The genes of chicken interleukin-2 (ChIL-2) and IBDV viral protein 2 (VP2) were amplified and fused by splice overlap extension-polymerase chain reaction (SOE-PCR). The fusion gene was digested by EcoR I/Kpn I and inserted into pBacPAK8 vector, resulting in recombinant transfer plasmid pBacPakVP2-IL2. The recombinant plasmid was transfected into Sf-9 cells accompanied with hybrid nuclear polyhedrosis virus (HyNPV) genome DNA and lipofectin. Plaque-purification indicated that we had got the recombinant Hy-VP2-IL2. Fusion protein VP2-IL2was expressed effectively both in insect cells and bombyx mori. The expression of fusion protein was confirmed by ELISA, SDS-PAGE and Western blotting assay, respectively. This efficient system allows us to meet the need for inexpensive vaccines required by the poultry industry. 关键词：ChlL-2 VP2溶解蛋白基因表达疫苗

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Preparation of ChlL-2 and IBDV VP2 Fusion Protein by Baculovirus Expression System

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