应用激光捕获显微切割分析深部子宫内膜异位症和位置正常子宫内膜的芳香酶及17β-羟类固酮脱氢酶2型mRNA表达

查看全文 作　　者：Matsuzaki [1]S.;Canis M.;Pouly J.-L.;张旸 机构地区：[1]Department of Gynecology, Polyclinique de l' Htel-Dieu, CHU, Clermont-Ferrand, 13 bd, L. Mafreyt, 63000, Clermont- Ferrand, France,Dr. 出　　处：《世界核心医学期刊文摘(妇产科学分册)》索引2006年第2卷第6期,共2页 摘　　要：Objective: To investigate mRNA expression of aromatase and 17β - hydroxysteroid dehydrogenase type 2 (17β HSD2) in epithelial and stromal cells from eutopic and ectopic endometrium of patients with deep endometriosis. Design: Prospective study. Setting: University hospital. Patient(s): Patients with deep endometriosis and fertile women with macroscopically normal pelvic cavities. Intervention(s): During surgery,30 endometrial and 16 endometriotic samples were obtained from 30 patients with deep endometriosis. Control endometrial samples were obtained from 24 fertile women with macroscopically normal pelvic cavities who underwent laparoscopic tubal ligation or reversal of tubal sterilization. Epithelial cells and stromal cells from endometrial or endometriotic tissues were microdissected using laser capture microdissection. Main Outcome Measure(s): Expression levels of aromatase and 17β HSD2 mRNA in microdissected epithelial and stromal cells were determined using quantitative real- time reverse transcriptase polymerase chain reaction. Result(s): Aromatase mRNA expression was significantly higher in epithelial cells than in stromal cells in both eutopic and ectopic endometrium obtained from endometriosis patients. In the ectopic endometrium of 8 patients (8/16, 50% ), 17β HSD2 expression was not detected in either epithelial or stromal cells. In eutopic endometrium from endometriosis patients, 17β HSD2 expression in epithelial cells was significantly increased during the early, middle, and late secretory phases compared with the late proliferative phase, whereas no significant cyclical difference was detected in control endometrium. Conclusion(s): Local estrogen concentration may be much higher in epithelial cells than in stromal cells in deep endometriotic tissue. 关 键 词：子宫内膜异位症 激光捕获显微切割 正常子宫内膜 MRNA表达 芳香酶 脱氢酶 β- 子宫内膜上皮