维普中文期刊产品整合服务

Polaprezinc protects human colon cells from oxidative injury induced by hydrogen peroxide:Relevant to cytoprotective heat shock proteins

查看全文 作　　者：Tatsuya [1]Ohkawara;Jun [2]Nishihira;Rika [3]Nagashima;Hiroshi [3]Takeda;Masahiro [3]Asaka 高影响力作者 机构地区：[1]Department of Gastroenterology and Hematology, Hokkaido University Graduate School of Medicine, Kita15, Nishi7, Kita-ku, Sapporo, 060-8678, Japan Department of Research and Development, GeneticLab Co. Ltd., Kita9, Nishi15, Chuou-ku, Sapporo, 060-0009, Japan;[2]Department of Research and Development, GeneticLab Co. Ltd., Kita9, Nishi15, Chuou-ku, Sapporo, 060-0009, Japan;[3]Department of Gastroenterology and Hematology, Hokkaido University Graduate School of Medicine, Kita15, Nishi7, Kita-ku, Sapporo, 060-8678, Japan高影响力机构 出　　处：《World Journal of Gastroenterology》索引2006年第12卷第38期,共4页高影响力期刊 基　　金：Supported by the Grant-in-Aid for research, No.18590665 from the Ministry of Education, Science and Culture of Japan 摘　　要：AIM: To investigate the effect of polaprezinc on cellular damage induced by hydrogen peroxide (H2O2) in human colon CaCo2 cells. METHODS: CaCo2 cells were treated with polaprezinc (10-100 μmol/L) for 6 h. After polaprezinc treatment, the cells were incubated with H2O2 (20 μmol/L) for 1 h. Cell viability was measured by MTT assay. Western blot analysis for heat shock protein (HSP) 27 and HSP72 in the cells was performed. Moreover, cells were pretreated with quercetin (200 μmol/L), an inhibitor of HSP synthe- sis, 2 h before polaprezinc treatment, and cell viability and the expression of HSP27 and 72 were assessed in these cells. RESULTS: Polaprezinc significantly protected CaCo2 cells from cell damage induced by H2O2, and up-regulated the expressions of HSP27 and HSP72 in the cells (10, 30 and 100 μmol/L of polaprezinc; 35.0% ± 7.7%, 58.3% ± 14.6% and 64.2% ± 8.2%, respectively. P < 0.01 versus polaprezinc-nontreated cells; 6.0% ± 4.4%). Quercetin inhibited the up-regulation of HSP27 and HSP72 by pola- prezinc and diminished the protective effect of polaprez- inc against H2O2-caused injury in the cells. CONCLUSION: Polaprezinc is a useful therapeutic agent for treatment of colitis and its effects depend on the function of cytoprotective HSP in colon. 关 键 词：结肠细胞 氧化损伤 过氧化氢 热休克蛋白
相关文献

参考文献(21)

引证文献(3)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费