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Early apoptosis and cell death induced by ATX-S10Na(Ⅱ)-mediated photodynamic therapy are Bax- and p53-dependent in human colon cancer cells

查看全文 作　　者：Makoto [1]Mitsunaga;Akihito [2]Tsubota;Kohichi [2]Nariai;Yoshihisa [2]Namiki;Makoto [2]Sumi;Tetsuya [2]Yoshikawa;Kiyotaka [1]Fujise 高影响力作者 机构地区：[1]Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Chiba, Japan Division of Gastro-enterology and Hepatology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo, Japan;[2]Institute of Clinical Medicine and Research, Jikei University School of Medicine, Kashiwa, Chiba, Japan高影响力机构 出　　处：《World Journal of Gastroenterology》索引2007年第13卷第5期,共7页高影响力期刊 基　　金：Supported by a grant from the Jikei University School of Medicine 摘　　要：AIM: To investigate the roles of Bax and p53 proteins in photosensitivity of human colon cancer cells by using lysosome-localizing photosensitizer, ATX-S10Na (Ⅱ).METHODS: HCT116 human colon cancer cells and Bax-null or p53-null isogenic derivatives were irradiated with a diode laser. Early apoptosis and cell death in response to photodynamic therapy were determined by MTT assays, annexin V assays, transmission electron microscopy assays, caspase assays and western blotting.RESULTS: Induction of early apoptosis and cell death was Bax- and p53-dependent. Bax and p53 were required for caspase-dependent apoptosis. The levels of anti-apoptotic Bcl-2 family proteins, Bcl-2 and Bcl-xL, were decreased in Bax- and p53-independent manner.CONCLUSION: Our results indicate that early apoptosis and cell death of human colon cancer cells induced by photodynamic therapy with lysosome-localizing photosensitizer ATX-S10Na (Ⅱ) are mediated by p53-Bax network and low levels of Bcl-2 and Bcl-xL proteins. Our results might help in formulating new therapeutic approaches in photodynamic therapy. 关 键 词：结肠癌 癌细胞 细胞凋亡 细胞死亡 光动力疗法 ATX-S10Na 介导 BAX p53
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