Inhibiting effect of melittin on pathogens of crops

查看全文 作　　者：PAN LingZi NA Jie XING Zhuo FANG HongJun WANG [1]GuanLin 机构地区：[1]College of Life Science, Liaoning Normal University, Dalian 116029, China 出　　处：《Chinese Science Bulletin》索引2007年第52卷第5期,共6页 基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30170112);the International Cooperation Project (Grant No. 30310103130) 摘　　要：The spectrum of antimicrobial effects of melittin were investigated on 19 pathogens by using a cylin-der-plate method with serial dilutions. Bacteriostatic efficiency and possible mechanistic effects were monitored via growth curves. The mechanism of inhibition was further analyzed by SDS-PAGE, flow cytometry and electron microscopy. Melittin had a wide inhibition spectrum and killed pathogens ef-fectively, and bacteriostatic action was influenced by factors such as pH and temperature. We eluci-dated three inhibitory mechanisms: melittin integrated with the cell membrane causing cell bursting and cytoplasm release, inhibited the synthesis of proteins and caused the cytoplasm to condense, and delayed pathogens in phase I (or phase G1) so that they could not complete the cell cycle. These re-sults suggest that melittin could serve as a broad-spectrum biological pesticide with fast-action and high-efficiency. 关 键 词：蜂毒肽 作物 病原菌 抑制作用 抑菌机制 抗菌谱