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Effects of aqueous extracts of Aconitum carmichaeli,Rhizoma bolbostemmatis,Phytolacca acinosa,Panax notoginseng and Gekko swinhonis Gūenther on Bel-7402 cells

查看全文 作　　者：Zhu-Chen [1]Yan;Dan [2]Chen;Xiong-Zhi [1]Wu;Guang-Ru [1]Xie;Yi [1]Ba;Zhao [1]Yan 高影响力作者 机构地区：[1]Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China;[2]Tianijn Infectious Diseases Hospital, Tianjin 300192,China高影响力机构 出　　处：《World Journal of Gastroenterology》索引2007年第13卷第19期,共4页高影响力期刊 摘　　要：AIM: To investigate the anticancer activity of a chinese medical mixture, WRCP (warming and relieving Cold Phlegm), on hepatocarcinoma Bel-7402 cells. METHODS: Fingerprints of WRCP, which were composed of aqueous extracts of Aconitum carmichaeli, Rhizoma bolbostemmatis, Phytolacca acinosa, Panax notoginseng and Gekko swinhonis Gūenther, and aconitine, which could be isolated from Aconitum carmichaeli and have the potential toxicity, were identified by high pressure liquid chromatography. Bel-7402 cells were grown in the presence of WRCP, As2O3 or all-trans-retinoic acid (ATRA). Cell proliferation and viability were determined by trypan blue stain. Apoptosis and cell cycle of Bel-7402 cells were detected by flow cytometry. Morphologic and ultrastructural variations were determined under optic and electronic microscopy. The secretion of alpha-fetoprotein and albumin was detected by radioimmunoassay. RESULTS: The average quality of aconitine is 1.15 ± 0.10 μg per 7.5 g extracts. WRCP could suppress the proliferation and viability of Bel-7402 cells. The percentage of apoptosis cells and S phase cells increased on WRCP-treated cells. Treated with WRCP, Bel-7402 cells showed ultrastructural features of differentiation. The alpha-fetoprotein secretion decreased while the albumin secretion increased (P < 0.001, P < 0.001, respectively) markedly in WRCP-treated cells.CONCLUSION: WRCP can affect the proliferation, differentiation and apoptosis of Bel-7402 cells. It can arrest cells in S phase and has strong cytotoxicity to Bel-7402 cells. 关 键 词：肝细胞癌 BEL-7402细胞 中草药水提物 细胞增殖 细胞分化 细胞凋亡 细胞毒性 乌头 贝母 三七 壁虎 商陆
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