Biological effect of expression of exogenous human nuclear receptor hLRH-1 in SW480 cells and its molecular mechanism

查看全文 作　　者：Shuiliang [1]Wang;Yingmeng [1,2]Zheng;Fenghua [1]Lan

机构地区：[1]Center for Molecular Medicine, Institute of Laboratory Medicine, Fuzhou General Hospital, Fuzhou 350025, China;[2]Department of Laboratory Medicine, First Hospital of Q.uanzhou, Q.uanzhou 362000, China

出　　处：《The Chinese-German Journal of Clinical Oncology》索引2007年第6卷第3期,共4页

基　　金：the Young Scientific and Technical Innovation Foundation of Fujian Province (No. 2004J067);Foundation of Fuzhou General Hospital (No. 200638) 摘　　要：Objective: To explore the possible biological function of human nuclear receptor hLRH-1 in tumorigenesis and progress of colon cancer. Methods: Plasmids pcDNA3-hLRH-1 were introduced into SW480 cells via lipofectamine. The expression of mRNA and protein of exogenous hLRH-1 were detected by RT-PCR and western blotting, respectively. MTT assay was carried out to survey the proliferation of SW480 cells with overexpression of hLRH-1. Meanwhile, the expression of proliferation-related genes cyclin E1 and cyclin D1, and apoptosis-related genes PTEN and Rb1, were analyzed by real- time RT-PCR. Results: The proliferation of SW480 cells was promoted under the condition of overexpression of hLRH-1. The expression of cyclin E1 was up-regulated significantly, while that of PTEN and Rb1 were down-regulated in SW480 cells with overexpressed hLRH-1. Conclusion: The expression of exogenous hLRH-1 in SW480 cells induced the proliferation resulting form up-regulation of cyclin E1, as well as participated in the regulation of apoptosis via influencing the expression of PTEN and Rb1. 关键词：生物治疗克隆技术癌细胞核磁疗法

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Biological effect of expression of exogenous human nuclear receptor hLRH-1 in SW480 cells and its molecular mechanism

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