Biocompatibility studies of silk fibroin-based artificial nerve grafts in vitro and in vivo

查看全文 作　　者：Chen Xuemei Yang Yumin Wu Jian Zhao Yahong Ding Fei Gu [1]Xiaosong

机构地区：[1]Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China

出　　处：《Progress in Natural Science:Materials International》索引2007年第17卷第9期,共6页

基　　金：Supported by National High-tech Research and Development Program(Grant No.2006AA02A128);National Natural Science Foundation ofChina(Grant No.30670667) 摘　　要：Silk fibroin(SF)has been used extensively in the biomedical field including tissue engineering for the generation of artificial bones,skins or ligaments.We have previously reported on good in vitro biocompatibility of SF fibers with peripheral nerve tissues and cells.In the present study,we developed a novel design of the SF-based artificial nerve graft(SF graft)which was composed of a SF-nerve guidance conduit(NGC)inserted with SF fibers.MTT assay was performed to determine the cytotoxicity of the SF-NGC extract fluid on the cultured L929 cells derived from an immortalized mouse fibroblast cell line.In addition,this SF graft was implanted into adult rats for bridging a 10-mm long sciatic nerve defect.The following-up experiments at initial stage(1-4 week)of nerve regeneration including routine blood tests and histochemical investigation were conducted to evaluate the in vivo biocompatibility of the SF graft with peripheral nerves.The results demonstrated that the SF-NGC graft was biocompatible with the surrounding tissues and cells due to its low inflammatory potential with a grade 0 under the U.S.Pharmacopeia guidelines and it was generally suitable to a certain degree for bridging peripheral nerve defects in virtue of supporting Schwann cell adherence,expansion and migration.Therefore the SF graft is a promising alternative to classical autografts for peripheral nerve repair. 关键词：生物适应性人造神经神经导管细胞毒性

相关文献

参考文献（10）
引证文献（5）
耦合文献（0）

参考文献(10)

引证文献(5)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Biocompatibility studies of silk fibroin-based artificial nerve grafts in vitro and in vivo

参考文献(10)

引证文献(5)

用户提示