Cloning and Sequence Analysis of a Full-length cDNA Encoding Light-harvesting Complexes of Photosystem II in Phyllostachys edulis

查看全文 作　　者：LIU [1]Yingli;GAO [1]Zhimin;PENG [2]Zhenhua;YUE [1]Yongde

机构地区：[1]International Center for Bamboo and Rattan Key Laboratory on Bamboo and Rattan Science and Technology of State Forestry Administration, Beijing 100102, P.R. China;[2]Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 P.R. China

出　　处：《Chinese Forestry Science and Technology》索引2007年第6卷第4期,共6页

基　　金：This project is supported by '948' Introduction Project (2004-4-60, 2005-4-38) 摘　　要：The light-harvesting chlorophyll a/b-protein complex plays an important role in photosynthesis of plants. A full-length cDNA of light-harvesting chlorophyll a/b (cab) gene was cloned from the first strand of Moso (Phyllostachys edulis) cDNA through RT-PCR and RACE methods, named as cab- PhE10 (cab gene 10 from Ph. edulis). The length of cab- PhE10 (GenBank accession number: EU118754) is 1 151 bp, which contains an open reading frame encoding 283 amino acids from 81st to 932nd position. The bioinformatics analysis indicated that the protein encoded by cab-PhE10 had a chlorophll a/b binding domain (83rd -247th position), two protein kinase C-phosphorylation sites, three N- myristoylation sites and a yia A/B double helix domain.The amino acid sequence of cab-PhE10 showed high similarity with the cab genes of Oryza sativa, Zea mays, Hordeum vulgare, and Vitis vinifera, more than 80%, respectively, which indicated that cab-PhE10 gene belongs to lhcb5 gene family. 关键词：脱氧核糖核酸克隆技术光合体系编码

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Cloning and Sequence Analysis of a Full-length cDNA Encoding Light-harvesting Complexes of Photosystem II in Phyllostachys edulis

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