Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

查看全文 作　　者：Shun [1]Chen;An-Chun [2]Cheng;Ming-Shu [2,3]Wang;Xi [2]Peng

机构地区：[1]Avian Diseases Research Centre, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China;[2]Avian Diseases Research Centre, College of Veterinary Medicine of Sichuan Agricultural University; Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Yaan 625014, Sichuan Province, China;[3]College of Life Science and Technology of Southwest University for Nationalities, Chengdu 610041, Sichuan Province, China

出　　处：《World Journal of Gastroenterology》索引2008年第14卷第14期,共5页

基　　金：The National Natural Science Foundation of China, No. 39970561;The Key Projects in the National Science and Technology Pillar Program, 2007Z06-017;Program for New Century Excellent Talents from Universities, Chinese Ministry of Education, No. NCET-04-0906/NCET-06-0818;Fund of the Discipline Leaders of Sichuan Province, No. SZD0418;Culture Fund for Excellent Doctoral Dissertations of Sichuan Agricultural University, 2008scybpy-1 摘　　要：AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/ PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non- apoptotic cells decreased. 关键词：成纤维细胞病毒性肠炎细胞凋亡治疗方法

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Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling

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