Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri

查看全文 作　　者：[1]张洋;[1]张晓军;Chantel F. [2]SCHEURING;[2]张洪斌;[1]李富花;[1]相建海

机构地区：[1]Institute of Oceanology,Chinese Academy of Sciences;[2]Department of Soil & Crop Sciences,Texas A&M University,College Station

出　　处：《Chinese Journal of Oceanology and Limnology》索引2008年第26卷第2期,共4页

基　　金：Key Program of National Natural Science Foundation of China (No. 30730071);Major State Basic Research Development Program of China (2006CB101804) 摘　　要：Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents. 关键词：BAC库扇贝基因组海洋开发

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Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri

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