Cloning and Analysis of Full-Length cDNA of PumNPR1 Gene from Pyrus ussuriensis Maxim

查看全文 作　　者：CHE [1,2]Daidi;FAN [2]Jinping;WANG [1,2]Jingang;XU [2]Ping;YANG [2]Tao;LIU [1]Shenkui

机构地区：[1]College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China;[2]College of Horticulture, Northeast Agricultural University, Harbin 150030, China

出　　处：《Journal of Northeast Agricultural University(English Edition)》索引2008年第15卷第2期,共6页

摘　　要：The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance.Pyrus ussuriensis Maxim is used as a starting material to clone the full-length cDNA of NPR1(nonexpressor of pathogenesis-related genes 1) which is a key regulator in SA(salicylic acid)-mediated systemic acquired resistance(SAR) by homologous cloning and RACE techniques.The length of the cDNA sequence was 1 767 bp,the ORF was 1 761 bp,it coded 586 amino acids,pI=5.58,the relative molecular weight was 65.009 ku,contained 19 kinds of amino acids,and had full BTB/POZ and ANK domains.Compared the homology of NPR1 gene in GenBank database,the homology with Pyrus pyrifolia,Arabidopsis thaliana,Nicotiana tabacum,Lycopersicon esculentum,Oryza sativa,Helianthus annuus were 98%,62%,68%,65%,57%,63%.The homology of functional area were 99%,78%,82%,79%,74%,77%.This NPR1 gene was considered as homologic gene of Pyrus ussuriensis Maxim and named PumNPR1. 关键词：基因克隆基因表达 NPR1 全长CDNA 蔷薇

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Cloning and Analysis of Full-Length cDNA of PumNPR1 Gene from Pyrus ussuriensis Maxim

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