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Analysis of SSRs Derived from an EST Library of Half-Smooth Tongue Sole Cynoglossus semilaevis

查看全文 作　　者：LIU [1,2]Yunguo;BAO [3]Baolong;CHEN [4]Songlin;SUN [5]Xiuqin 高影响力作者 机构地区：[1]College of Fisheries, Ocean Universitv of China, Qingdao 266003, P. R. China;[2]Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries ResearchInstitute, Shanghai 200090, P. R. China;[3]College of Aqua-Life ; Science and Technology, Shanghai Ocean University, Shanghai 200090, P. R. China;[4]Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, P. R. China;[5]The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, P. R. China高影响力机构 出　　处：《Journal of Ocean University of China》索引2008年第7卷第3期,共4页高影响力期刊 基　　金：This work was supported by grants from the Key Laboratory of Marine and Estuarine Fisheries,the Ministry of Agriculture(05-03-01);the Key Laboratory of Aquatic Genetic Resources and Aquaculmral Ecology(AGRA)Certified by the Ministry of Agriculture(KFT2006-5);Shandong Postdoctoral Science Foundation(200601001);China Postdoctoral Science Foundation(20060390999). 摘　　要：A complementary DNA(cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag(EST) database was generated,containing 3100 cDNA clones,of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats(SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats(51.4%) are the most abundant class of SSR in the dataset. The dimeric,trimeric,tetrameric and hexameric re-peats are represented in decreasing proportions of 27.2%,16.0%,2.8% and 1.9%,respectively. The frequency of pentameric repeats was observed the least(only 0.7%) . Most of the monomeric and dimeric repeats are distributed in 3' and 5' un-translation region. If translation regions are considered merely,trimeric repeats are the highest,accounting for 57% of the total microsatellites. 关 键 词：半滑舌鳎 分析方法 基因 鱼
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