Expression of the SOD gene from Trichoderma harzianum in Saccharomyces cerevisiae

查看全文 作　　者：[1,2]杨力明;Yang [1]Qian;Liu [1]Pigang;Li [1]Sen

机构地区：[1]Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, P.R. China;[2]Department of Pathophysiology, Harbin Medical University, Harbin 150001, P.R. China

出　　处：《High Technology Letters》索引2008年第14卷第3期,共5页

基　　金：the National High Technology Research and Development Programme of China(No.2003AA241140) 摘　　要：Superoxide dismutases are metalloproteins which play a major role in defense against oxygen radical-mediated toxicity in aerobic organisms.Such proteins are important endogeneity cytoprotection factor in-volving defence.A 751-bp full-length cDNA sequence of an SOD gene was isolated from the Tr/chodermaharzianum.The full-length cDNA of the SOD gene consists of one 465-bp open reading frame nu-cleotide,which encodes a 15.7-kDa polypeptide consisting of 154 amino acid residues.Sequence analy-sis revealed that SOD gene has more than 72%-86% amino acid sequence homology with those of otherfungi.The SOD gene was integrated into the genomic DNA of pYES2 by insertion into a single site for re-combination,yielding the recombinant pYES2-SOD.SOD expressed by pYES2-SOD was induced bygalactose.We test whether SOD could offer abiotic stress resistance when it was introduced into yeastcells.A transgenic yeast harboring T.harzianum SOD was generated under the control of a constitutivelyexpressed GAL promoter.The results indicated that SOD yeast transformants had significantly higher re-sistance to salt and drought stress. 关键词：SOD基因基因表达渗透压力转基因酵母

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Expression of the SOD gene from Trichoderma harzianum in Saccharomyces cerevisiae

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