Bacterial Community Structure and Diversity in a Black Soil as Affected by Long-Term Fertilization

查看全文 作　　者：WEI [1,2]Dan;YANG [1]Qian;ZHANG Jun-[1]Zheng;WANG [1]Shuang;CHEN Xue-[2]Li;ZHANG Xi-[2]Lin;LI Wei-[2]Qun

机构地区：[1]Harbin Institute of Technology, Harbin 150001 (China).;[2]The Institute of Soil Fertility and Environmental Sources, Heilongjiang Academy of Agricultural Sciences, Harbin 150086(China)

出　　处：《Pedosphere》索引2008年第18卷第5期,共11页

基　　金：the Heilongjiang Provincial Natural Science Funds for Distinguished Young Scholars, China (No.JC200622);the Heilongjiang Provincial Natural Science Foundation of China (No.C2004-17);the National 'Eleventh Five Years Plan' Key Project on Science and Technology of China (No.2006BAD25B05). 摘　　要：Black soil(Mollisol) is one of the main soil types in northeastern China. Biolog and polymerase chain reactiondenaturing gradient gel electrophoresis(PCR-DGGE) methods were used to examine the infiuence of various fertilizer combinations on the structure and function of the bacterial community in a black soil collected from Harbin,Heilongjiang Province. Biolog results showed that substrate richness and catabolic diversity of the soil bacterial community were the greatest in the chemical fertilizer and chemical fertilizer+manure treatments. The metabolic ability of the bacterial community in the manure treatment was similar to the control. DGGE fingerprinting indicated similarity in the distribution of most 16S rDNA bands among all treatments,suggesting that microorganisms with those bands were stable and not infiuenced by fertilization. However,chemical fertilizer increased the diversity of soil bacterial community. Principal component analysis of Biolog and DGGE data revealed that the structure and function of the bacterial community were similar in the control and manure treatments,suggesting that the application of manure increased the soil microbial population,but had no effect on the bacterial community structure. Catabolic function was similar in the chemical fertilizer and chemical fertilizer+manure treatments,but the composition structure of the soil microbes differed between them. The use of chemical fertilizers could result in a decline in the catabolic activity of fast-growing or eutrophic bacteria. 关键词：黑土长期施肥菌群结构化学肥料

相关文献

参考文献（37）
引证文献（47）
耦合文献（290）

参考文献(37)

引证文献(47)

耦合文献(290)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Bacterial Community Structure and Diversity in a Black Soil as Affected by Long-Term Fertilization

参考文献(37)

引证文献(47)

耦合文献(290)

用户提示