金克诱导Molt-4细胞凋亡及其机制研究(英文)

查看全文 作　　者：Zhenchuang Zhu Yixin Tong Yangping Yue Yu Deng Dongdong Yu Wei Xiao Xiaolan Li Deding Tao Junbo Hu Jianping [1]Gong

机构地区：[1]Molecular Medical Center, Cancer Research Institute, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

出　　处：《The Chinese-German Journal of Clinical Oncology》索引2008年第7卷第8期,共5页

基　　金：grants of Foundation of '973' program (No. 2004CB518705);National Natural Science Foundation of China (No. 30570908) 摘　　要：Objective: To investigate the apoptosis-inducing effect of Jinke on Molt-4 cells and its possible mechanism. Methods: The Molt-4 cells were treated with different concentrations of Jinke and then cultured for necessary time. The An- nexin-V / PI method was used to detect the apoptosis rate. The cell cycle was analyzed by DNA content with flow cytometry. Double parameters analysis of cyclins / DNA was performed to detect the expression of cyclin E. API method was used to confirm the cell cycle-specific apoptosis. The expressions of Bcl-2 and Bax were detected by western blot. Results: 24 h after the treatment of 0.5, 1.0, 1.5, 2.0 and 3.0 mg/mL Jinke, the apoptosis rate of Molt-4 cells was evaluated in a concentra- tion-dependent manner, from 5.2% of the control group to 41.0% of the 3.0 mg/mL Jinke group. When the Molt-4 cells were cultured with 1.5 mg/mL Jinke, the apoptosis rate was evaluated in a time-dependent manner. DNA content analysis showed that G0/G1 phase of Molt-4 cells increased in a time-dependent manner. The expression of cyclin E increased gradually. API assay showed the apoptosis cells were almost in G0/G1 phase. Western blot showed the Bcl-2 was down-regulated and the Bax was up-regulated. Conclusion: Jinke could induce G1 phase-specific apoptosis in Molt-4 cells in time- and concentra- tion-dependent manners involving G1 phase arrest. The mechanism of apoptosis inducing effect may be related to the up- regulation of Bax and the down-regulation of Bcl-2. 关键词：金克诱导 MOLT-4 细胞凋亡机制研究

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金克诱导Molt-4细胞凋亡及其机制研究(英文)

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