Laboratory Characterizations on 2007 Cases of Monoclonal Gammopathies in East China

查看全文 作　　者：Hao [1]Wang;Chunfang [2]Gao;Lingling [1]Xu;Zaixing [1]Yang;Wenjing [1]Zhao;Xiantao [1]Kong

机构地区：[1]Department of Laboratory Medicine, Changzheng Hospital, SecondMilitary Medical University, Shanghai 200003, China;[2]Department of Laboratory Medicine, Eastern Hepatobiliary Hospital,Second Military Medical University. Shanghai 200438. China

出　　处：《Cellular & Molecular Immunology》索引2008年第5卷第4期,共6页

基　　金：Natural Science Foundation of China No.30270605. 摘　　要：Monoclonal gammopathies are characterized by the presence of monoclonal immunoglobulin in patients with or without evidence of multiple myeloma (MM),macroglobulinemia,amyloidosis (AL),or a related plasma cell proliferative disorder. This study aims to evaluate laboratory diagnostic characters of monoclonal gammopathies and investigates the correlation between monoclonal gammopathies and transforming growth factor β1 (TGFβ1). Immunofixation electrophoresis (IFE),serum protein electrophoresis (SPE),nephelometry and urine light chain ELISA were used for laboratory identification of monoclonal immunoglobulins. Plasma TGFβ1 was detected with double-antibodies ELISA. Lightcycler was used for single nucleotide polymorphism (SNP) analysis. Totally 2,007 cases of monoclonal immunoglobulin (M protein) were identified in 10,682 samples. The isotypes of M protein were IgG type 47.1%,IgA 23.0%,IgM 8.7%,IgD 5.3%,free light chain κ 6.1%,λ 9.8%. In reference to IFE,the coherency of diagnosis was serum light chain ratio (κ/λ) 94.4%,quantitation of Igs 83%,light chain quantitation 80.9%,and urine light chain ratio (κ/λ) 58.0%. Plasma TGFβ1 was elevated significantly compared to normal control. The allelic frequency of codon 10 (C > T) was neither associated with the existence of the M protein nor with the M protein isotype. Monoclonal gammopathies can be identified with the combination of IFE,SPE,Igs quantitaion and urine light chain determination. Although TGFβ1,an important cytokine in immune regulation,was elevated in monoclonal gammopathies,the SNPs in coding region of TGFβ1 gene did not confer susceptibility to the development of monoclonal gammopathies in this study. 关键词：实验分析免疫球蛋白骨髓瘤巨球蛋白血症淀粉样变性病浆细胞无序扩散

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Laboratory Characterizations on 2007 Cases of Monoclonal Gammopathies in East China

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