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Comparison of the chloride channel activator lubiprostone and the oral laxative Polyethylene Glycol 3350 on mucosal barrier repair in ischemic-injured porcine intestine

查看全文 作　　者：Adam J [1]Moeser;Prashant K [2]Nighot;Birgit [3]Roerig;Ryuji [3]Ueno;Anthony T [2]Blikslager 高影响力作者 机构地区：[1]Department of Population Health and Pathobiology,College of Veterinary Medicine,North Carolina State University,Raleigh NC 27606,United States;[2]Department of Clinical Sciences,College of Veterinary Medicine,North Carolina State University,Raleigh NC 27606,United States;[3]Sucampo Pharmaceuticals,Inc,Bethesda MD 20814,United States高影响力机构 出　　处：《World Journal of Gastroenterology》索引2008年第14卷第39期,共6页高影响力期刊 摘　　要：AIM: To investigate the effects of lubiprostone and Polyethylene Glycol 3350 (PEG) on mucosal barrier repair in ischemic-injured porcine intestine. METHODS: Ileum from 6 piglets (approximately 15 kg body weight) was subjected to ischemic conditions by occluding the local mesenteric circulation for 45 min in vivo. Ileal tissues from each pig were then harvested and mounted in Ussing chambers and bathed in oxygenated Ringer's solution in vitro. Intestinal barrier function was assessed by measuring transepithelial electrical resistance (TER) and mucosal-to-serosal fluxes of 3H-mannitol and 14C-inulin. Statistical analyses of data collected over a 120-min time course included 2-way ANOVA for the effects of time and treatment on indices of barrier function. RESULTS: Application of 1 μmol/L lubiprostone to the mucosal surface of ischemic-injured ileum in vitro induced significant elevations in TER compared to non-treated tissue. Lubiprostone also reduced mucosal-to-serosal fluxes of 3H-mannitol and 14C-inulin. Alternatively,application of a polyethylene laxative (PEG,20 mmol/L) to the mucosal surface of ischemic tissues significantly increased flux of 3H-mannitol and 14C-inulin. CONCLUSION: This experiment demonstrates that lubiprostone stimulates recovery of barrier function in ischemic intestinal tissues whereas the PEG laxative had deleterious effects on mucosal repair. These results suggest that,unlike osmotic laxatives,lubiprostone stimulates repair of the injured intestinal barrier. 关 键 词：肠缺血 渗透率 泻药 聚乙烯
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