Effect of Different Fertilizer Treatments on Quantity of Soil Microbes and Structure of Ammonium Oxidizing Bacterial Community in a Calcareous Purple Paddy Soil

查看全文 作　　者：GU Yun-[1]fu;YUN [1]Xiang;ZHANG Xiao-[1]ping;TU Shi-[2]hua;SUN Xi-[2]fa;Kristina [3]Lindstrom

机构地区：[1]College of Resource and Environment, Sichuan Agricultural University, Ya'an 625014, P.R.China;[2]Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, P.R.China;[3]Department of Applied Chemistry and Microbial, University of Helsinki, SF-00014, Finland

出　　处：《Agricultural Sciences in China》索引2008年第7卷第12期,共9页

基　　金：the National Key Technology R&D Program of China (2006BAD05B06 and 2006BAD 02A14-13) 摘　　要：The quantity of soil microbes and the structure of ammonium oxidizing bacterial (AOB) community were analyzed using the dilution plate counting and most probable number method (MPN), and denaturing gradient gel electrophoresis (DGGE), respectively. Fertilizer application tended to increase the number of soil microbes and alter the AOB community compared to the control with no fertilizer application (CK). Among the eight fertilizer treatments, soil samples from the treatments of mineral fertilizers (e.g., N, P, K) in combination with farmyard manure (M) had greater numbers of soil microbes and more complex structure of AOB community than those receiving mineral fertilizers alone. The principal component analyses (PCA) for ammonium oxidizing bacterial community structure showed that the eight fertilizer treatments could be divided into two PCA groups (PCA1 and PCA2). For the soil sampled after rice harvest, PCA1 included NP, NM, NPM and NPKM fertilizer treatments, while PCA2 was consisted of CK, N, M and NPK fertilizer treatments. For soil samples collected after wheat harvest, PCA1 was consisted of M, NM, NPM and NPKM fertilizer treatments, while PCA2 was composed of CK, N, NP and NPK fertilizer treatments. For a given rotation, the richness of AOB community in PCA1 was greater than that in PCA2. In addition, AOB community structure was more complex in the soil after rice harvest than that after wheat harvest. The results indicated that different fertilizer treatments resulted in substantial changes of soil microbe number and AOB community. Furthermore, mineral fertilizers (N, NP, NPK) combined with farmyard manure were effective for increasing the quantity of soil microbes, enriching AOB community, and improving the soil biofertility. 关键词：土壤长期施肥石灰质微生物化学合成

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Effect of Different Fertilizer Treatments on Quantity of Soil Microbes and Structure of Ammonium Oxidizing Bacterial Community in a Calcareous Purple Paddy Soil

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