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Molecular characterization and expression analysis of a chicken-type lysozyme gene from housefly (Musca domestica)

查看全文 作　　者：Qian Ren, Xiaofan Zhao, Jinxing Wang School of Life Sciences, Shandong University, Jinan 250100, China 高影响力作者 出　　处：《Journal of Genetics and Genomics》索引2009年第36卷第1期,共10页高影响力期刊 基　　金：supported by the National High Technology Research and Development Program of China (863 Program) (No. 2007AA09Z425 and 2006AA100311);the Ph.D. Program Foundation of the Ministry of Education of China (No. 20060422034). 摘　　要：Lysozymes can hydrolyze bacteria and play an important role in animal digestion and innate immunity. The cDNA of a chicken-type lysozyme gene (Mdlys) was cloned from housefly (Musca domestica). The 484 bp full-length cDNA contains a 426 bp open reading frame (ORF) that encodes MdLys of 141 amino acids. Phylogenetic analysis indicated that the MdLys was similar to chicken-type lysozymes. Spatio-temporal expression of Mdlys was analyzed by RT-PCR. The Mdlys transcript can be detected in both midgut and fat body and was expressed at a relatively lower level at the embryo stage. Mdlys mRNA was upregulated 2 h post bacterial challenge, main-tained for 2 to 6 h, and slightly declined from 12 to 24 h post-injection. Western blot analysis showed that MdLys was highly expressed in midgut and was also detected in the hemolymph and fat body. MdLys expression was slightly increased in midgut after challenging with Escherichia coli or Staphylococcus aureus. Its expression was also slightly increased in the fat body after challenging with S. aureus, but no obvious change occurred after E. coli challenge. MdLys expression in the hemolymph was not affected by bacterial challenge. In the developmental stages, MdLys expression levels had no obvious change from the first instar to the pupae stage. There was also no varia-tion under 24 h starvation stress. Recombinant MdLys displayed inhibitory activity against Gram-negative and Gram-positive bacteria. Together, these results suggest that MdLys may play an important role in the innate immunity of houseflies. 关 键 词：免疫遗传学 遗传基因 自然免疫 溶解酵素 抗菌免疫性
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