Mapping of two new brown planthopper resistance genes from wild rice

查看全文 作　　者：WANG [1]Buna;HUANG [1]Zhen;SHU [1]Lihui;REN [1]Xiang;LI [2]Xianghua;HE [1]Guangcun

机构地区：[1]Key Laboratory for Plant Developmental Biology,College of Life Science,Wuhan University,Wuhan 430072,China;[2]National Key Laboratory of Genetic Improvement of Crop,Huazhong Agricultural University,Wuhan 430064,China

出　　处：《Chinese Science Bulletin》索引2001年第46卷第13期,共5页

基　　金：This work was supported by the State '863' High-Tech Program. 摘　　要：A brown planthopper (BPH) resistance line, B5, derived its resistance genes from the wild rice Oryza offici-nalis Wall exwatt, was hybridized with Taichung Native 1, a cultivar highly susceptible to BPH. A mapping population composed of randomly selected 167 F2 individuals was used for determining the BPH resistance genes by the restriction fragment length polymorphism analysis (RFLP). Bulked segregant analysis was conducted to identify RFLP makers linked to the BPH resistance genes in B5. The results indicated that the markers linked to BPH resistance are located at two genomic regions on the long arm of chromosome 3 and the short arm of chromosome 4, respectively. The existence of the two loci was further assessed by the quantitative trait locus (QTL) analysis. We located the two loci at a 3.2 cM interval between G1318 and R1925 on chromosome 3 and a 1.2 cM interval between C820 and S11182 on chromosome 4. Comparison with the BPH genes that have been reported indicated that the BPH resistance genes 关键词：ORYZA SALIVA L. BROWN PLANTHOPPER resistance GENE molecular marker GENE localization.

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Mapping of two new brown planthopper resistance genes from wild rice

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