Differential gene expression of protein kinases in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp

查看全文 作　　者：Jing Xie;Yan Zhu;Fan Zhang;Jianfang Gui

机构地区：[1]Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China;[2]Wuhan Univ, Wuhan, Peoples R China;

出　　处：《Chinese Science Bulletin》索引1999年第44卷第14期,共5页

摘　　要：A modified mRNA differential display method has been applied to studying differential expression of protein kinase genes in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp. Total RNA was reverse transcribed using downstream 3′primers T₁₂MA, T₁₂MG and T₁₂MC respectively. Then the reverse transcription products were amplified using upstream 5′kinase-specific primer designed according to protein kinase conserved sequence. The PCR products had different patterns and numbers of cDNA bands on polyacrylamide gel. Totally 21 cDNAs fragments were recovered and cloned. Two of them were confirmed to be particularly expressed in oocytes of amphimictic crucian carp, and another was specific for gynogenetic silver crucian carp. 关键词：mRNA differential DISPLAY GYNOGENESIS protein KINASE SILVER crucian carp.

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Differential gene expression of protein kinases in oocytes between natural gynogenetic silver crucian carp and amphimictic crucian carp

参考文献(16)

引证文献(5)

耦合文献(8)

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