Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significanly correlated with cytochrome P450 suppression

查看全文 作　　者：Xia Chen Chang-Kai Sun Guo-Zhu Han Jin-Yong Peng Ying Li Yan-Xia Liu Yuan-Yuan Lv Ke-Xin Liu Qin Zhou Hui-Jun [1]Sun

机构地区：[1]Department of Pharmacology, Daiian Medical University, Dalian 116044, Liaoning Province, China

出　　处：《World Journal of Gastroenterology》索引2009年第15卷第15期,共7页

基　　金：Grant from the Science Foundation of Educational Department of Liaoning Province,05L117;Dalian Science&Technology Bureau,2007J22JH012 摘　　要：AIM:To investigate the hepatoprotective activity of tea polyphenols(TP)and its relation with cytochrome P450(CYP450)expression in mice. METHODS:Hepatic CYP450 and CYPb5 levels were measured by UV-spectrophotometry in mice 2 d after intraperitoneal TP(25,50 and 100 mg/kg per day). Then the mice were intragastricly pre-treated with TP (100,200 and 400 mg/kg per day)for six days before paracetamol(1000 mg/kg)was given.Their acute mortality was compared with that of control mice. The mice were pre-treated with TP(100,200,and 400 mg/kg per day)for five days before paracetamol (500 mg/kg)was given.Hepatic CYP2E1 and CYP1A2 protein and mRNA expression levels were evaluated by Western blotting,immunohistochemical staining and transcriptase-polymerase chain reaction. RESULTS:The hepatic CYP450 and CYPb5 levels in mice of TP-treated groups(100,200 and 400 mg/kg per day)were decreased in a dose-dependent manner compared with those in the negative control mice.TP significantly attenuated the paracetamol-induced hepatic injury and dramatically reduced the mortality of paracetamol-treated mice.Furthermore,TP reduced CYP2E1 and CYP1A2 expression at both protein and mRNA levels in a dose-dependent manner. 关键词：肝细胞色素P450 对乙酰氨基酚小鼠肝损伤茶多酚保护作用 CYP2E1基因免疫组织化学染色紫外分光光度法

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Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significanly correlated with cytochrome P450 suppression

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