Components of the mitogen-activated protein kinase cascade are activated in hepatic cells by Echinococcus multilocularis metacestode

查看全文 作　　者：Ren-Yong [2]Lin;Jun-Hua [1]Wang;Xiao-Mei [1]Lu;Xiao-Tao [1]Zhou;Georges [3]Mantion;Hao [1]Wen;Dominique A [4]Vuitton;Lysiane [2]Richert

机构地区：[1]Xinjiang Key Laboratory on Echinococcosis and Liver Surgery, 1st Teaching Hospital of Xinjiang Medical University, No. 1 Liyushan Road, Urumqi 830054, China;[2]Ren-Yong Lin, Lysiane Richert, Laboratoire de Toxicologie Cellulaire, EA 4267, Faculte de Medecine et Pharmacie, University of Franche-Comte, Place Saint-Jacques, 25030 Besangon, France;[3]Liver Surgery and Transplantation Unit, EA 3921, Department of Digestive Surgery, University Hospital Jean Minjoz, Boulevard Fleming, 25030 Besangon, France; WHO-Collaborating Centre for the Prevention and Treatment of Human Echinococcosis, University of Franche-Comte, Place Saint Jacques, 25030 Besancon, France;[4]WHO-Collaborating Centre for the Prevention and Treatment of Human Echinococcosis, University of Franche-Comte, Place Saint Jacques, 25030 Besancon, France

出　　处：《World Journal of Gastroenterology》索引2009年第15卷第17期,共9页

基　　金：Supported by A PhD grant from the French Ministry of Foreign Affairs (French Embassy in Beijing) to Ren-Yong Lin;by a project grant from the 'Foundation Transplantation' (2005-2006);by a grant from NSFC, No. 30860253 and 30760239;by the Xinjiang Key-Lab project grants on Echinococcosis, No. XJDX0202-2005-01 and XJDX0202-2007-04 摘　　要：AIM: To explore the effect of Echinococcus multilocularis (E. multilocularis) on the activation of mitogen-activated protein kinase (MAPK) signaling pathways and on liver cell proliferation. METHODS: Changes in the phosphorylation of MAPKs and proliferating cell nuclear antigen (PCNA) expression were measured in the liver of patients with alveolar echinococcosis (AE). MAPKs, MEK1/2 [MAPK/ extracellular signal-regulated protein kinase (ERK) kinase] and ribosomal S6 kinase (RSK) phosphorylation were detected in primary cultures of rat hepatocytes in contact in vitro with (1) E. multilocularis vesicle fluid (EmF), (2) E. multilocularis-conditioned medium (EmCM). RESULTS: In the liver of AE patients, ERK 1/2 and p38 MAPK were activated and PCNA expression was increased, especially in the vicinity of the metacestode. Upon exposure to EmF, p38, c-Jun N-terminal kinase (JNK) and ERK1/2 were also activated in hepatocytes in vitro, as well as MEK1/2 and RSK, in the absence of any toxic effect. Upon exposure to EmCM, only JNK was up-regulated. CONCLUSION: Previous studies have demonstrated an influence of the host on the MAPK cascade in E. multilocularis. Our data suggest that the reverse, i.e. parasite-derived signals efficiently acting on MAPK signaling pathways in host liver cells, is actually operating. 关键词：丝裂原活化蛋白激酶多房棘球绦虫肝细胞激活细胞外信号调节蛋白激酶增殖细胞核抗原分裂原 P38激酶

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Components of the mitogen-activated protein kinase cascade are activated in hepatic cells by Echinococcus multilocularis metacestode

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