Lower Bifidobacteria counts in both duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients

查看全文 作　　者：Angèle PM [1]Kerckhoffs;Melvin [1]Samsom;Michel E van der [2]Rest;Joris de [2]Vogel;Jan [3]Knol;Kaouther Ben-[3]Amor;Louis MA [1]Akkermans

机构地区：[1]Gastrointestinal Research Unit, Department of Gastroenterology and Surgery, University Medical Center Utrecht, Heidelberglaan 100, F02.618, 3584CX, Utrecht, The Netherlands;[2]BioVisible BV, LJ Zielstraweg 1, 9713 GX, Groningen, The Netherlands;[3]Danone Research-Centre for Specialized Nutrition, Bosrandweg 20, 6704PH, Wageningen, The Netherlands

出　　处：《World Journal of Gastroenterology》索引2009年第15卷第23期,共6页

摘　　要：AIM: To determine the composition of both fecal and duodenal mucosa-associated microbiota in irritable bowel syndrome (IBS) patients and healthy subjects using molecular-based techniques. METHODS: Fecal and duodenal mucosa brush samples were obtained from 41 IBS patients and 26 healthy subjects. Fecal samples were analyzed for the composition of the total microbiota using fluorescent in situ hybridization (FISH) and both fecal and duodenal brush samples were analyzed for the composition of bif idobacteria using real-time polymerase chain reaction. RESULTS: The FISH analysis of fecal samples revealed a 2-fold decrease in the level of bifidobacteria (4.2 ± 1.3 vs 8.3 ± 1.9, P < 0.01) in IBS patients compared to healthy subjects, whereas no major differences in other bacterial groups were observed. At the species level, Bifidobacterium catenulatum levels were significantly lower (6 ± 0.6 vs 19 ± 2.5, P < 0.001) in the IBS patients in both fecal and duodenal brush samples than in healthy subjects.CONCLUSION: Decreased bifidobacteria levels in both fecal and duodenal brush samples of IBS patients compared to healthy subjects indicate a role for microbiotic composition in IBS pathophysiology. 关键词：微生物利用双歧杆菌肠粘膜综合征粪便患者微生物组成样本分析

相关文献

参考文献（49）
引证文献（33）
耦合文献（0）

参考文献(49)

引证文献(33)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Lower Bifidobacteria counts in both duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients

参考文献(49)

引证文献(33)

用户提示