Effect of growth hormone on small intestinal homeostasis relation to cellular mediators IGF-I and IGFBP-3

查看全文 作　　者：Betul [1]Ersoy;Kemal [2]Ozbilgin;Erhun [3]Kasirga;Sevinc [4]Inan;Senol [5]Coskun;Ibrahim [4]Tuglu

机构地区：[1]Division of Pediatric Endocrinology,Department of Pediatrics,Faculty of Medicine,Celal Bayar University, Manisa 45010,Turkey;[2]Department of Histology and Embryology, Faculty of Medicine,Celal Bayar University,Manisa 45010, Turkey;[3]Division of Pediatric Gastroenterology,De- partment of Pediatrics,Faculty of Medicine,Celal Bayar University,Manisa 45010,Turkey;[4]Department of Histology and Embryology,Faculty of Medicine,Celal Bayar University, Manisa 45010,Turkey;[5]Department of Pediatrics,Faculty of Medicine, Celal Bayar University,Manisa 45010,Turkey

出　　处：《World Journal of Gastroenterology》索引2009年第15卷第43期,共7页

摘　　要：AIM:To evaluate the effects of growth hormone(GH) on the histology of small intestines which might be related to the role of insulin like growth factor(IGF)-I, IGF-binding protein 3(IGFBP-3)and its receptors. METHODS:Twelve week-old adult male Wistar albino rats were divided into two groups.The study group(n =10),received recombinant human growth hormone (rGH)at a dose of 2 mg/kg per day subcutaneously for 14 d and the control group(n=10)received physiologic serum.Paraffin sections of jejunum were stained with periodic acid shift(PAS)and hematoxylin and eosin(HE) for light microscopy.They were also examined for IGF-I, IGFBP-3 and IGF-receptor immunoreactivities.Staining intensity was graded semi-quantitatively using the HS- CORE. RESULTS:Goblet cells and the cells in crypt epitheliawere significantly increased in the study group compared to that of the control group.We have demonstrated an increase of IGF-I and IGFBP-3 immunoreactivities in surface epithelium of the small intestine by GH application.IGF-I receptor immunoreactivities of crypt,villous columnar cells,enteroendocrine cells and muscularis mucosae were also more strongly positive in the study group compared to those of in the control group. CONCLUSION:These findings confirm the important trophic and protective role of GH in the homeostasis of the small intestine.The trophic effect is mediated by an increase in IGF-I synthesis in the small intestine, but the protective effect is not related to IGF-I. 关键词：重组人生长激素 IGFBP 小肠细胞平衡关系胰岛素样生长因子结合蛋白细胞免疫反应生长因子受体调解

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Effect of growth hormone on small intestinal homeostasis relation to cellular mediators IGF-I and IGFBP-3

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