p75 neurotrophin receptor signal pathway influence on apoptosis in anterior horn neurons of the spinal cord in a rat model of cauda equina compression injury

查看全文 作　　者：Haopeng Li Jie Chen Xijing He Siyue Xu Yu [1]Liu

机构地区：[1]Department of Orthopaedics, Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China

出　　处：《Neural Regeneration Research》索引2009年第4卷第12期,共7页

基　　金：the National Natural Science Foundation of China, No. 30672136 摘　　要：BACKGROUND: Studies have demonstrated that cauda equina compression results in apoptosis ofmotor neurons in the spinal cord. The combination of p75 neurotrophin receptor (p75NTR) andprecursor of nerve growth factor (pro-NGF) expression initiates the apoptotic pathway and inducesneuronal apoptosis. However, few reports have focused on the p75-mediated mechanism ofneuronal apoptosis following cauda equine compression injuryOBJECTIVE: To determine apoptosis of spinal cord neurons and activation of thepro-NGF-p75NTR-JNK(c-Jun N-terminal kinase) signal pathway in rats following cauda equinacompression, and to verify experimental outcomes.DESIGN, TIME AND SETTING: A randomized, controlled, in vivo experiment was performed at theMedical Experimental Center of Xi'an Jiaotong University between April and November in 2008.MATERIALS: Streptavidin-perosidase kit was purchased from Wuhan Boster, China; in situ endlabeling detection kit was provided by Promega, USA; type AEG-220G electron microscope waspurchased from Hitachi, Japan.METHODS: A total of 48 healthy, adult, female, Sprague Dawley rats were randomly assigned tothree groups: normal (n = 6), sham-surgery (n = 6), and compression (n = 36). The compressiongroup was randomly assigned to six subsets at 1,3, 5, 7, 14, and 28 days, respectively, with 6 rats ineach subset. A cylindrical silica gel stick was implanted into the rats to compress 75% of thevertebral canal in the compression group; in the sham-surgery group, only vertebral resection wasperformed; and no procedures were performed in the normal group.MAIN OUTCOME MEASURES: At 1,3, 5, 7, 14, and 28 days following compression, L_(2-3) spinalcord segments were processed for immunohistochemistry, in situ cell apoptosis detection, andtransmission electron microscopy observation. Nissl staining was used to observe neuronal survivalin the L_2 spinal cord segment. Immunohistochemistry was applied to detect expressions of pro-NGF,p75NTR, and JNK in the L_2 segment. TUNEL fluorometric method was used to observe apoptosis ofneurons in the L_2 segment.RESULTS: In the normal and sham-surgery groups, little neuronal apoptosis was observed in theL_(2-3) spinal cord segment. At 3 days after compression injury, pro-NGF, p75NTR and JNK expressionwas observed in the spinal cord. Expression levels reached a peak at 7 days, and then graduallydecreased. In the compression and sham-surgery groups, neurons primarily expressed pro-NGFand p75NTR. The number of JNK-positive neurons in the compression group was dramaticallyincreased compared with the sham-surgery group (P < 0.05). A few neurons were apoptotic in thespinal cord 1 day after compression injury. The number of apoptotic neurons gradually increasedand reached a peak at 7 days, and subsequently decreased. Apoptosis was still detectable at 28days. There was a positive correlation between p75NTR expression and neuronal apoptosis(r= 0.75, P< 0.05).CONCLUSION: Following cauda equina compression injury, apoptosis of spinal cord neurons wasobserved. The compression-induced neuronal apoptosis was associated with p75NTR expression inthe L_(2-3) spinal cord segment. 关键词：神经细胞凋亡 P75NTR 神经营养素受体脊髓神经元压缩损伤信号通路大鼠模型神经营养因子

相关文献

参考文献（25）
引证文献（1）
耦合文献（18）

参考文献(25)

引证文献(1)

耦合文献(18)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

p75 neurotrophin receptor signal pathway influence on apoptosis in anterior horn neurons of the spinal cord in a rat model of cauda equina compression injury

参考文献(25)

引证文献(1)

耦合文献(18)

用户提示