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过表达人血管生成素样蛋白4对食管癌EC9706细胞生长的影响(英文)

查看全文 作　　者：Shangen [1]Zheng;Fang [2]Wang;Yinjuan [1]Ding;Lifang [1]Zhang;Qianchuan [1]Huang;Guoqiang [3]Zhao 高影响力作者 机构地区：[1]Department of Transfusion, Wuhan General Hospital of Guangzhou Millitary Command, Wuhan 430070, China;[2]Health Department, Air Force Radar Academy, Wuhan 430019, China;[3]Department of Microbiology, Basic Medical Science School, Zhengzhou University, Zhengzhou 450000, China高影响力机构 出　　处：《The Chinese-German Journal of Clinical Oncology》索引2010年第9卷第2期,共5页高影响力期刊 摘　　要：Objective:The aim of the study was to construct the eukaryotic expression vector of human angiopoietin-like protein 4(ANGPTL4) and observe the effect of ANGPTL4 overexpression on the growth of esophageal carcinoma EC9706 cells.Methods:Total RNA was extracted from normal hepatic tissue,and ANGPTL4 cDNA was amplified by RT-PCR.The PCR product was doubly digested by XbaI and SalI,and then recombined into eukaryotic expression vector.Then,pIRES-GFP-ANGPTL4 was obtained by G418 selection,then pIRES-GFP-ANGPTL4 and pIRES-GFP were transfected into EC9706 cells with lipidosome-packaged method.Meanwhile,the transfected cells were selected by G418,and then stable transfected cell lines were obtained.ANGPTL4 mRNA levels,the cell cycles and growth curves of EC9706 cells in experiment group(transfected with pIRES-GFP-ANGPTL4),empty vector group(transfected with pIRES-GFP) and blank control group(EC9706 cells without transfection) were detected with RT-PCR,flow cytometry and MTT methods,respectively.Results:Eukaryotic ANGPTL4 expression vector pIRES-GFP-ANGPTL4 was successfully constructed.The ANGPTL4 mRNA level(0.21 ± 0.03) in experiment group was significantly higher than that of the empty vector group(0.04 ± 0.008) and the blank control group(0.05 ± 0.007),with significant differences(P < 0.01).The proportion of cells in S phase in experiment group was significantly different with those of the other two groups(P < 0.05).The cell growth of EC9706 cells in experiment group was slower than those of the other two groups.From the third day,the differences began to be significant.Conclusion:ANGPTL4 overexpression in esophageal carcinoma EC9706 cells could inhibit the growth of EC9706 cells. 关 键 词：血管生成素 过度表达 细胞生长 食管癌 蛋白
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