Determination of Protein via Resonance Light Scattering Technique with (NH_4)_2SO_4

查看全文 作　　者：WU Li-[1]hang;MU [1]Dan;LIANG Fang-[2]hui;TIAN [1]Yuan;ZHANG Han-[1]qi;YU Ai-[1]min;WANG Xing-[1]hua;CHEN [3]Ying

机构地区：[1]College of Chemistry,Jilin University,Changchun 130012,P.R.China;[2]Department of Pharmaceutical Science,Changchun Medical College,Changchun 130031,P.R.China;[3]Jilin Province Center of Environmental Monitoring,Changchun 130011,P.R.China

出　　处：《Chemical Research in Chinese Universities》索引2010年第26卷第2期,共5页

摘　　要：Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering(RLS),(NH4)2SO4 was used as a RLS probe to determine bovine serum albumin(BSA),human serum albumin(HSA) and ovum albumin(OVA).The experimental conditions were investigated,including the acidity and saturation degree of (NH4)2SO4.Under the optimum conditions,the enhanced RLS intensity is proportional to the concentration of BSA,HSA and OVA in the ranges of 5×10-8―1×10-6,2.5×10-8―8×10-7 and 5×10-8―1.5×10-6 mol/L,respectively.The detection limits for BSA,HAS and OVA are 6.6×10-9,3.8×10-9 and 7.4×10-9 mol/L,respectively.The effects of foreign substances were also examined.The practical and synthetic samples were analyzed with satisfactory results. 关键词：(NH4)2SO4 共振光散射技术高蛋白质牛血清白蛋白人血清白蛋白共振光散射探针测定静电作用

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Determination of Protein via Resonance Light Scattering Technique with (NH_4)_2SO_4

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