Uptake of transferrin-conjugated quantum dots in single living cells

查看全文 作　　者：[1,2]陈丹妮;[2]许改霞;Bahi Ahmed [3,4]Ali;Ken-Tye [5]Yong;[3]周翠红;[3]王晓梅;[2]屈军乐;Paras [6]N.Prasad;[2]牛憨笨 机构地区：[1]College of Optoelectronic Science and Engineering, Huazhong University of Science and Technology;[2]Institute of Optoelectronics, Key Lab of Optoelectronics Devices and Systems of Ministry of Education/Guangdong Province, Shenzhen Key Lab of Biomedicine Engineering, Shenzhen University;[3]College of Medicine, Shenzhen University;[4]Mubarak City for Scientific Research and Technology Applications;[5]School of Electrical and Electronic Engineering, Nanyang Technological University;[6]Institute for Laser, Photonics and Biophotonics, State University of New York at Buffalo 出　　处：《Chinese Optics Letters》索引2010年第8卷第10期,共4页 基　　金：supported by the National Natural Science Foundation of China (Nos. 30900335, 60878053, and60627003);the Guangdong Province Science Foundation(No. 2008078);the Natural Science Foundation of Shen-zhen University, and the Start-Up Grant from Nanyang Technological University 摘　　要：We study the uptake and distribution of transferrin (Tf)-conjugated CdSe/CdS/ZnS quantum dots (QDs) in single living HeLa cells with both fluorescence confocal microscopy and three-dimensional (3D) reconstruction technique. By increasing the co-incubation time or the dosage of QDs-Tf, we find that the uptake of QDs-Tf bioconjugates in the cells increases correspondingly, but with different uptake rates. Additionally, the distribution of QDs-Tf, in single live HeLa cells is time dependent. To our knowledge, this is the first study on quantitatively analyzing the uptake and distribution of bioconjugated QDs in single living cells. Such QDs nanoplatform can be further modified for developing biomedical evaluation tool in cancer diagnosis and targeted drug delivery. 关 键 词：转铁蛋白 量子点 吸收率 活细胞 标记 HeLa细胞 时间依赖性 共聚焦显微镜