Ultrastructure of neuronal-like cells differentiated from adult adipose-derived stromal cells

查看全文 作　　者：Changqing Ye Xiaodong Yuan Hui Liu Yanan [1]Cai

机构地区：[1]Department of Neurology, Kailuan General Hospital, North China Coal Medical University, Tangshan 063000, Hebei Province, China

出　　处：《Neural Regeneration Research》索引2010年第5卷第19期,共8页

摘　　要：β-mercaptoethanol induces in vitro adult adipose-derived stromal cells(ADSCs) to differentiate into neurons.However,the ultrastructural features of the differentiated neuronal-like cells remain unknown.In the present study,inverted phase contrast microscopy was utilized to observe β-mercaptoethanol-induced differentiation of neuronal-like cells from human ADSCs,and immunocytochemistry and real-time polymerase chain reaction were employed to detect expression of a neural stem cells marker(nestin),a neuronal marker(neuron-specific enolase),and a glial marker(glial fibrillary acidic protein).In addition,ultrastructure of neuronal-like cells was observed by transmission election microscopy.Results revealed highest expression rate of nestin and neuron-specific enolase at 3 and 5 hours following induced differentiation;cells in the 5-hour induction group exhibited a neuronal-specific structure,i.e.,Nissl bodies.However,when induction solution was replaced by complete culture medium after 8-hour induction,the differentiated cells reverted to the fibroblast-like morphology from day 1.These results demonstrate that β-mercaptoethanol-induced ADSCs induced differentiation into neural stem cells,followed by morphology of neuronal-like cells.However,this differentiation state was not stable. 关键词：神经元样细胞超微结构特征细胞分化基质细胞神经元特异性烯醇化酶脂肪来源成人胶质纤维酸性蛋白

相关文献

参考文献（34）
引证文献（16）
耦合文献（192）

参考文献(34)

引证文献(16)

耦合文献(192)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Ultrastructure of neuronal-like cells differentiated from adult adipose-derived stromal cells

参考文献(34)

引证文献(16)

耦合文献(192)

用户提示