Isobaric Tags for Relative and Absolute Quantificationbased Comparative Proteomics Reveals the Features of Plasma Membrane-Associated Proteomes of Pollen Grains and Pollen Tubes from Lilium davidii

查看全文 作　　者：Bing [1,4]Han;Sixue [2]Chen;Shaojun [3]Dai;Ning [1,4]Yang;Tai [1]Wang

机构地区：[1]Research Center for Molecular & Developmental Biology, Key Laboratory of Photosynthesis & Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and National Center for Plant Gene Research, Beijing 100093, China;[2]Department of Biology, Genetics Institute and Interdisciplinary Center for Biotechnology Research, University of Florida, GaJnesville, Florida 32610, USA;[3]Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Northeast Forestry University, Harbin 150040, China;[4]Graduate University of the Chinese Academy of Sciences, Beijing 10049, China

出　　处：《Journal of Integrative Plant Biology》索引2010年第52卷第12期,共16页

基　　金：supported by the Chinese Ministry of Science and Technology (2007CB947601);the Chinese Academy of Sciences (KSCX2-YW-N-026);the New Century Excellent Talents in Chinese Universities (NECT-06-0327);Fundamental Research Funds for the Central Universities (DL09DA03),China;faculty startup funds from the University of Florida 摘　　要：Mature pollen grains (PGs) from most plant species are metabolically quiescent. However, once pollinated onto stigma, they quickly hydrate and germinate. A PG can give rise to a vegetative cell-derived polarized pollen tube (PT), which represents a specialized polar cell. The polarized PT grows by the tip and requires interaction of different signaling molecules localized in the apical plasma membrane and active membrane trafficking. The mechanisms underlying the interaction and membrane trafficking are not well understood. In this work, we purified PG and PT plasma-membrane vesicles from Lilium davidii Duch. using the aqueous two-phase partition technique, then enriched plasma membrane proteins by using Brij58 and KCl to remove loosely bound contaminants. We identified 223 integral and membrane-associated proteins in the plasma membrane of PGs and PTs by using isobaric tags for relative and absolute quantification (iTRAQ) and 2-D high-performance liquid chromatography-tandem mass spectrometry. More than 68% of the proteins have putative transmembrane domains and/or lipid-modified motifs. Proteins involved in signal transduction, membrane trafficking and transport are predominant in the plasma-membrane proteome. We revealed most components of the clathrin-dependent endocytosis pathway. Statistical analysis revealed 14 proteins differentially expressed in the two development stages: in PTs, six upregulated and eight downregulated are mainly involved in signaling, transport and membrane trafficking. These results provide novel insights into polarized PT growth. 关键词：比较蛋白质组学等离子体膜花粉管百合标签比重高效液相色谱信号转导

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Isobaric Tags for Relative and Absolute Quantificationbased Comparative Proteomics Reveals the Features of Plasma Membrane-Associated Proteomes of Pollen Grains and Pollen Tubes from Lilium davidii

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